Zhu Yan, Wei Qianhui, Jin Qingxi, Li Gangrong, Zhang Qingzhu, Xiao Han, Li Tengfei, Wei Feng, Luo Yingchun
School of Chemical Engineering, Guizhou Minzu University, Guiyang 550025, China.
State Key Laboratory of Advanced Materials for Smart Sensing, GRINM Group Co., Ltd., Beijing 100088, China.
Nanomaterials (Basel). 2023 Feb 2;13(3):604. doi: 10.3390/nano13030604.
Accurate monitoring of blood glucose levels is crucial for the diagnosis of diabetes patients. In this paper, we proposed a simple "mixed-catalyzer layer" modified silicon nanowire field-effect transistor biosensor that enabled direct detection of glucose with low-charge in high ionic strength solutions. A stable screening system was established to overcome Debye screening effect by forming a porous biopolymer layer with polyethylene glycol (PEG) modified on the surface of SiNW. The experimental results show that when the optimal ratio (APTMS:silane-PEG = 2:1) modified the surface of silicon nanowires, glucose oxidase can detect glucose in the concentration range of 10 nM to 10 mM. The sensitivity of the biosensor is calculated to be 0.47 μAcmmM, its fast response time not exceeding 8 s, and the detection limit is up to 10 nM. This glucose sensor has the advantages of high sensitivity, strong specificity and fast real-time response. Therefore, it has a potential clinical application prospect in disease diagnosis.
准确监测血糖水平对于糖尿病患者的诊断至关重要。在本文中，我们提出了一种简单的“混合催化剂层”修饰的硅纳米线场效应晶体管生物传感器，该传感器能够在高离子强度溶液中以低电荷直接检测葡萄糖。通过在硅纳米线表面形成聚乙二醇（PEG）修饰的多孔生物聚合物层，建立了一个稳定的筛选系统以克服德拜筛选效应。实验结果表明，当以最佳比例（APTMS：硅烷 - PEG = 2：1）修饰硅纳米线表面时，葡萄糖氧化酶能够检测浓度范围为10 nM至10 mM的葡萄糖。该生物传感器的灵敏度计算为0.47 μAcmmM，其快速响应时间不超过8秒，检测限高达10 nM。这种葡萄糖传感器具有灵敏度高、特异性强和实时响应快的优点。因此，它在疾病诊断方面具有潜在的临床应用前景。
