Weyand E H, Rice J E, LaVoie E J
Naylor Dana Institute for Disease Prevention, American Health Foundation, Valhalla, NY 10595.
Cancer Lett. 1987 Nov;37(3):257-66. doi: 10.1016/0304-3835(87)90110-8.
DNA adduct formation in vivo in mouse skin following a single topical application of benzo[a]fluoranthene (BbF), benzo[j]fluoranthene (BjF), benzo[k]fluoranthene (BkF), or indeno[1,2,3-cd]pyrene (IP) was investigated in female CD-1 mice using 32P-postlabeling analysis. Distinct adduct profiles were detected for each of the non-alternant hydrocarbons examined. Two adducts, one major and one minor, were detected using polyethyleneiminecellulose (PEI-cellulose) thin-layer chromatography (TLC) for BbF and BjF while a single major adduct was detected for BkF and IP. The relative extent of binding to mouse skin DNA was in the order BbF greater than BjF greater than BkF greater than IP. 32P-Postlabeled DNA adducts separated by PEI-cellulose TLC were further analyzed by high performance liquid chromatography (HPLC). A single radioactive peak was detected for 32P-labeled DNA adducts of BjF and BkF. Three general areas of radioactivity were detected when 32P-labeled DNA adducts of BbF were separated on HPLC.
使用³²P后标记分析法，在雌性CD - 1小鼠中研究了单次局部应用苯并[a]荧蒽（BbF）、苯并[j]荧蒽（BjF）、苯并[k]荧蒽（BkF）或茚并[1,2,3 - cd]芘（IP）后小鼠皮肤体内DNA加合物的形成情况。在所检测的每种非交替烃中均检测到了不同的加合物谱。使用聚乙烯亚胺纤维素（PEI - 纤维素）薄层层析（TLC）对BbF和BjF检测到两种加合物，一种主要加合物和一种次要加合物，而对BkF和IP检测到一种主要加合物。与小鼠皮肤DNA结合的相对程度顺序为BbF大于BjF大于BkF大于IP。通过PEI - 纤维素TLC分离的³²P后标记DNA加合物进一步通过高效液相色谱（HPLC）进行分析。对BjF和BkF的³²P标记DNA加合物检测到一个放射性峰。当BbF的³²P标记DNA加合物在HPLC上分离时，检测到三个放射性的一般区域。
