Ritz Manfred, Ahmad Nadim, Brueck Thomas, Mehlmer Norbert
Werner Siemens Chair of Synthetic Biotechnology, Department of Chemistry, Technical University of Munich (TUM), 85748 Garching, Germany.
Plants (Basel). 2023 Feb 1;12(3):632. doi: 10.3390/plants12030632.
, also known as bluebeard, is an ornamental plant containing a large variety of terpenes and terpene-like compounds. Four different cultivars were subjected to a principal component analysis to elucidate variations in terpenoid-biosynthesis and consequently, two representative cultivars were sequenced on a genomic level. Functional annotation of genes as well as comparative genome analysis on long read datasets enabled the identification of cultivar-specific terpene synthase and cytochrome p450 enzyme sequences. This enables new insights, especially since terpenoids in research and industry are gaining increasing interest due to their importance in areas such as food preservation, fragrances, or as active ingredients in pharmaceutical formulations. According to BUSCO assessments, the presented genomes have an average size of 355 Mb and about 96.8% completeness. An average of 52,090 genes could be annotated as putative proteins, whereas about 42 were associated with terpene synthases and about 1340 with cytochrome p450 enzymes.
,也被称为蓝胡子,是一种含有多种萜类和类萜化合物的观赏植物。对四个不同的品种进行了主成分分析,以阐明萜类生物合成的变化,因此,在基因组水平上对两个代表性品种进行了测序。基因的功能注释以及对长读数据集的比较基因组分析,使得能够鉴定出品种特异性的萜烯合酶和细胞色素P450酶序列。这带来了新的见解,特别是因为萜类化合物在研究和工业中由于其在食品保存、香料或作为药物制剂中的活性成分等领域的重要性而越来越受到关注。根据BUSCO评估,所呈现的基因组平均大小为355 Mb,完整性约为96.8%。平均有52,090个基因可以被注释为假定蛋白质,而约42个与萜烯合酶相关,约1340个与细胞色素P450酶相关。