Lee S, Gilula N B, Warner A E
Department of Anatomy and Embryology, University College London, England.
Cell. 1987 Dec 4;51(5):851-60. doi: 10.1016/0092-8674(87)90108-5.
The ability of gap junction antibodies to block dye transfer and electrical coupling was examined in the compacted 8-cell mouse zygote. In control zygotes, Lucifer yellow injected into 1 cell transferred to the rest of the embryo. When antibodies raised against the major protein extracted from gap junctions were co-injected with Lucifer yellow, dye transfer failed in 86% of the zygotes tested and electrical coupling was almost completely inhibited. Subsequently, the antibody-containing cells were extruded. When the antibodies were injected into 1 cell at the 2-cell stage, 82% of the zygotes divided normally to the 8-cell stage. Cells containing gap junction antibodies were uncompacted, but continued to divide. We conclude that these antibodies inhibit gap junctional communication in the early mouse zygote and that communication through gap junctions may be involved in the maintenance of compaction.
在致密化的8细胞小鼠受精卵中检测了间隙连接抗体阻断染料转移和电偶联的能力。在对照受精卵中，注入1个细胞的荧光黄会转移到胚胎的其他部分。当将针对从间隙连接中提取的主要蛋白质产生的抗体与荧光黄共同注入时，在86%的受试受精卵中染料转移失败，并且电偶联几乎被完全抑制。随后，含有抗体的细胞被挤出。当在2细胞阶段将抗体注入1个细胞时，82%的受精卵正常分裂至8细胞阶段。含有间隙连接抗体的细胞未致密化，但继续分裂。我们得出结论，这些抗体抑制早期小鼠受精卵中的间隙连接通讯，并且通过间隙连接的通讯可能参与致密化的维持。
