花粉授精后拟南芥柱头细胞细胞骨架重构。

Cytoskeleton Remodeling in Arabidopsis Stigmatic Cells Following Pollination.

机构信息

The Sainsbury Laboratory, Bateman Street, CB2 1LR, University of Cambridge, Cambridge, UK.

Laboratoire Reproduction et Developpement des Plantes, Universite de Lyon, ENS de Lyon, CNRS, INRAE, Lyon, France.

出版信息

Methods Mol Biol. 2023;2604:215-225. doi: 10.1007/978-1-0716-2867-6_16.

DOI:10.1007/978-1-0716-2867-6_16

PMID:36773236

Abstract

In plants, the first interaction that occurs between the male gametophytes (pollen grains) and the stigmatic epidermis of the female organ is crucial for successful reproduction. The stigma consists of a dome of flask-shaped cells specialized in pollen capture. In these stigmatic cells, the cytoskeleton network (cortical microtubules and actin microfilaments) actively responds to pollen contact and undergoes dynamic remodeling required for successful pollen acceptance to occur. Here, we have designed several microscopy mountings to monitor stigmatic cytoskeleton dynamics. These designs are based on the constraints linked to the tightly regulated pollen-stigma interaction and depend upon the experimental goal, either a static view or live-cell imaging.

摘要

在植物中，雄配子体（花粉粒）与雌性器官柱头表皮之间的第一次相互作用对于成功繁殖至关重要。柱头由专门用于捕获花粉的瓶状细胞组成。在这些柱头细胞中，细胞骨架网络（皮质微管和肌动蛋白微丝）对花粉接触做出积极响应，并进行动态重塑，这是成功接受花粉所必需的。在这里，我们设计了几种显微镜安装来监测柱头细胞骨架的动态。这些设计基于与严格调控的花粉-柱头相互作用相关的约束条件，并取决于实验目标，是静态观察还是活细胞成像。