Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing, China.
School of Life Sciences, Qilu Normal University, Jinan, China.
Methods Mol Biol. 2023;2604:285-295. doi: 10.1007/978-1-0716-2867-6_23.
The actin cytoskeleton plays an essential role in the regulation of polarized pollen tube growth, and its functions are dictated by its spatial organization and dynamics. Here we describe an assay to monitor the dynamics of actin filaments decorated with Lifeact-mEGFP in Arabidopsis pollen tubes using spinning disk confocal microscopy and measuring the parameters associated with their dynamics. The method allows us to assess the dynamics of actin filaments in growing Arabidopsis pollen tubes.
肌动蛋白细胞骨架在调节极化花粉管生长中起着至关重要的作用，其功能取决于其空间组织和动态。在这里，我们描述了一种使用旋转盘共聚焦显微镜监测用 Lifeact-mEGFP 标记的肌动蛋白丝在拟南芥花粉管中动态的测定法，并测量了与其动态相关的参数。该方法使我们能够评估在生长中的拟南芥花粉管中肌动蛋白丝的动态。
