Cell Therapy and Regenerative Medicine Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
Iranian Cancer Control Center (MACSA), Tehran, Iran.
Methods Mol Biol. 2024;2736:127-137. doi: 10.1007/7651_2022_475.
Diabetes mellitus can be categorized as one of the prolonged metabolic disorders that are associated with inappropriately elevated blood glucose levels. Among the subgroups of this disease, type 2 diabetes accounts for the most patients. Although pharmaceutical and non-pharmaceutical treatments have been employed to control the progression of the disease, as with any treatment approach, both therapeutic approaches are associated with side effects and challenges. Nowadays, the emergence of treatment methods based on stem cells has attracted the attention of researchers in order to treat diabetes fundamentally and provide a long-term solution. Since there are still blind spots regarding the positive and negative effects of these types of treatments, animal studies can give researchers a detailed insight into the effects of stem cell-based treatments. Recently, zebrafish has been proposed as a valuable animal model due to its outstanding genetic and physiological characteristics in biomedical studies including diabetes. Hereupon, in this protocol, the development and validation of type 2 diabetic zebrafish model for cell-based treatments have been explained.
糖尿病可归类为一种长期的代谢紊乱疾病，其特征是血糖水平异常升高。在该疾病的亚组中，2 型糖尿病占大多数患者。尽管已经采用了药物和非药物治疗来控制疾病的进展，但与任何治疗方法一样，这两种治疗方法都有副作用和挑战。如今，基于干细胞的治疗方法的出现引起了研究人员的关注，以期从根本上治疗糖尿病并提供长期解决方案。由于这些治疗方法的积极和消极影响仍存在盲点，因此动物研究可以让研究人员更详细地了解基于干细胞的治疗的效果。最近，由于其在包括糖尿病在内的生物医学研究中的出色遗传和生理特性，斑马鱼被提议作为一种有价值的动物模型。因此，在本方案中，解释了用于细胞治疗的 2 型糖尿病斑马鱼模型的开发和验证。
