School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China; Academy of Contemporary Food Engineering, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou 510006, China; Engineering and Technological Research Centre of Guangdong Province on Intelligent Sensing and Process Control of Cold Chain Foods, & Guangdong Province Engineering Laboratory for Intelligent Cold Chain Logistics Equipment for Agricultural Products, Guangzhou Higher Education Mega Centre, Guangzhou 510006, China.
School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China; Academy of Contemporary Food Engineering, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou 510006, China; Engineering and Technological Research Centre of Guangdong Province on Intelligent Sensing and Process Control of Cold Chain Foods, & Guangdong Province Engineering Laboratory for Intelligent Cold Chain Logistics Equipment for Agricultural Products, Guangzhou Higher Education Mega Centre, Guangzhou 510006, China; Food Refrigeration and Computerized Food Technology (FRCFT), Agriculture and Food Science Centre, University College Dublin, National University of Ireland, Belfield, Dublin 4, Ireland. Electronic address: http://www.ucd.ie/refrig.
Food Chem. 2023 Jun 30;412:135332. doi: 10.1016/j.foodchem.2022.135332. Epub 2022 Dec 26.
Cellulose material holds considerable promise for effective surface-enhanced Raman scattering (SERS) substrate construction due to its extensive availability, chemically modifying capacity, ease of manufacture, high flexibility and low optical activity. A large-area, high-sensitivity, stable and uniform Au@Ag nanorods (NRs)-CMC substrate was successfully developed via electrostatic repulsion by using negatively-charged core-shell Au@Ag NRs as SERS active plasmonic nanomaterial, combined with negatively-charged carboxymethylcellulose (CMC) hydrogel for nanoparticles stabilization, homodisperse and protection. The obtained Au@Ag NRs-CMC substrate showed excellent sensitivity for the detection of thiram residues in fruits containing low and abundant pigment interferents, such as apples and blueberries, with detection limits of 58 and 78 ppb, respectively. Additionally, it retained more than 80% SERS performance after storage for 9 months under ambient conditions, demonstrating its great potential in facilitating the commercialization of cellulose-based SERS technology for cost-effective detection of food contaminants with advantages of facile preparation procedure, uniformity, reproducibility and long-term stability.
纤维素材料由于其广泛的可用性、化学修饰能力、易于制造、高灵活性和低光活性，在有效构建表面增强拉曼散射（SERS）基底方面具有很大的潜力。通过使用带负电荷的核壳 Au@Ag NRs 作为 SERS 活性等离子体纳米材料，并结合带负电荷的羧甲基纤维素（CMC）水凝胶来稳定、均分散和保护纳米颗粒，成功地通过静电排斥作用开发出了大面积、高灵敏度、稳定且均匀的 Au@Ag 纳米棒（NRs）-CMC 基底。所获得的 Au@Ag NRs-CMC 基底对含有低含量和丰富色素干扰物（如苹果和蓝莓）的水果中的 thiram 残留具有出色的检测灵敏度，检测限分别为 58 和 78 ppb。此外，它在环境条件下储存 9 个月后仍保留超过 80%的 SERS 性能，这表明它在促进基于纤维素的 SERS 技术商业化方面具有很大的潜力，可用于以具有制备程序简便、均匀性、重现性和长期稳定性的优势来经济有效地检测食品污染物。
