Faculty of Engineering and Natural Sciences, Sabanci University, Istanbul, Türkiye; Department of Computational Science - Biological Sciences, TÜBİTAK Research Institute for Fundamental Sciences, Gebze, Türkiye.
Department of Biochemistry and Biophysics, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina, USA.
J Biol Chem. 2023 Mar;299(3):103009. doi: 10.1016/j.jbc.2023.103009. Epub 2023 Feb 11.
In vitro and in vivo experiments with Escherichia coli have shown that the Mfd translocase is responsible for transcription-coupled repair, a subpathway of nucleotide excision repair involving the faster rate of repair of the transcribed strand than the nontranscribed strand. Even though the mfd gene is conserved in all bacterial lineages, there is only limited information on whether it performs the same function in other bacterial species. Here, by genome scale analysis of repair of UV-induced cyclobutane pyrimidine dimers, we find that the Mfd protein is the transcription-repair coupling factor in Mycobacterium smegmatis. This finding, combined with the inverted strandedness of UV-induced mutations in WT and mfdE. coli and Bacillus subtilis indicate that the Mfd protein is the universal transcription-repair coupling factor in bacteria.
在大肠杆菌的体外和体内实验表明，Mfd 转位酶负责转录偶联修复，这是核苷酸切除修复的一个亚途径，涉及转录链比非转录链更快的修复速度。尽管 mfd 基因在所有细菌谱系中都保守，但关于它在其他细菌物种中是否具有相同功能的信息有限。在这里，通过对紫外线诱导的环丁烷嘧啶二聚体修复的基因组规模分析，我们发现 Mfd 蛋白是分枝杆菌中的转录修复偶联因子。这一发现，加上 WT 和 mfdE. coli 和枯草芽孢杆菌中紫外线诱导突变的反向链极性，表明 Mfd 蛋白是细菌中通用的转录修复偶联因子。
