Division of Biotechnology, College of Environmental and Bioresource Sciences, Jeonbuk National University, Iksan, Jeonbuk, South Korea.
Department of Geosciences, University of Tübingen, Schnarrenbergstraße 94-96, Tübingen 72076, Germany.
Sci Total Environ. 2023 May 10;872:162180. doi: 10.1016/j.scitotenv.2023.162180. Epub 2023 Feb 11.
Biofilms consist of single or multiple species of bacteria embedded in extracellular polymeric substances (EPSs), which affect the increase in antibiotic resistance by restricting the transport of antibiotics to the bacterial cells. An alternative approach to treatment with antimicrobial agents is using biofilm inhibitors that regulate biofilm development without inhibiting bacterial growth. In this study, we found that citrus peel extract from Jeju Island (CPEJ) can inhibit bacterial biofilm formation. According to the results, CPEJ concentration-dependently reduces biofilm formation without affecting bacterial growth. Additionally, CPEJ decreased the production of extracellular polymeric substances but increased bacterial swarming motility. These results led to the hypothesis that CPEJ can reduce intracellular bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP) concentration. The results showed that CPEJ significantly reduced the c-di-GMP level through increased phosphodiesterase activity. Altogether, these findings suggest that CPEJ as a biofilm inhibitor has new potential for pharmacological (e.g. drug and medication) and industrial applications (e.g. ship hulls, water pipes, and membrane processes biofouling control).
生物膜由单个或多种细菌嵌入细胞外聚合物（EPS）中组成，这会限制抗生素向细菌细胞的运输，从而增加抗生素耐药性。另一种对抗生素药物治疗的方法是使用生物膜抑制剂，它可以调节生物膜的发展，而不会抑制细菌的生长。在这项研究中，我们发现来自济州岛的柑橘皮提取物（CPEJ）可以抑制细菌生物膜的形成。结果表明，CPEJ 浓度依赖性地降低生物膜的形成，而不影响细菌的生长。此外，CPEJ 减少了胞外聚合物的产生，但增加了细菌的泳动运动性。这些结果表明，CPEJ 可以降低细胞内双-(3'-5')-环二鸟苷单磷酸（c-di-GMP）的浓度。结果表明，CPEJ 通过增加磷酸二酯酶活性显著降低了 c-di-GMP 水平。总的来说，这些发现表明，作为一种生物膜抑制剂，CPEJ 具有新的药理学（如药物和药物治疗）和工业应用（如船体、水管和膜过程生物污损控制）的潜力。
