Dhx15 通过 Wnt 信号通路调控斑马鱼肠道发育。

Dhx15 regulates zebrafish intestinal development through the Wnt signaling pathway.

机构信息

Union Clinical Medical Colleges, Fujian Medical University, Fuzhou, China.

Union Clinical Medical Colleges, Fujian Medical University, Fuzhou, China; Department of Hematology, Fujian Institute of Hematology, Fujian Provincial Key Laboratory on Hematology, Fujian Medical University Union Hospital, Fuzhou, China.

出版信息

Genomics. 2023 Mar;115(2):110578. doi: 10.1016/j.ygeno.2023.110578. Epub 2023 Feb 10.

DOI:10.1016/j.ygeno.2023.110578

PMID:36775210

Abstract

DEAH-box helicase 15 (DHX15) is ATP-dependent RNA helicase which is known for its role in RNA metabolism. Recent studies reported DHX15 involves in the intestinal immunity. However, the role of DHX15 (or RNA helicase) in intestinal development is poorly understood. Here, we revealed an unidentified role for dhx15 in regulating zebrafish intestinal development. We found the profound intestinal defects in dhx15 knockout zebrafish. Decreased proliferation and increased apoptosis of the intestine cells were observed when dhx15 were deleted. Further RNA genome wide analysis and qRT-PCR analysis showed the Wnt signaling pathway is down-regulated in the dhx15 knockout zebrafish. Thus, we concluded that dhx15 regulates zebrafish intestinal development through the Wnt signaling pathway. Here, we provided new insights into the role of dhx15 in intestinal development beyond its well-characterized role in intestinal immunity.

摘要

DEAH-box 解旋酶 15（DHX15）是一种依赖于 ATP 的 RNA 解旋酶，其在 RNA 代谢中发挥作用。最近的研究报道 DHX15 参与肠道免疫。然而，DHX15（或 RNA 解旋酶）在肠道发育中的作用知之甚少。在这里，我们揭示了 dhx15 在调节斑马鱼肠道发育中的一个未被识别的作用。我们发现 dhx15 敲除斑马鱼存在严重的肠道缺陷。当 dhx15 缺失时，观察到肠细胞增殖减少和凋亡增加。进一步的 RNA 全基因组分析和 qRT-PCR 分析表明，dhx15 敲除斑马鱼中的 Wnt 信号通路被下调。因此，我们得出结论，dhx15 通过 Wnt 信号通路调节斑马鱼肠道发育。在这里，我们提供了 dhx15 在肠道发育中的作用的新见解，超越了其在肠道免疫中特征明确的作用。