Mousazadeh Navid, Seidi Farzad, Ghaffarlou Mohammadreza, Rashidzadeh Hamid, Hosseinmirzaei Ali, Mozafari Faezeh, Danafar Hossein, Nosrati Hamed
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources and International Innovation Center for Forest Chemicals and Materials, Nanjing Forestry University, Nanjing 210037, China; Zanjan Pharmaceutical Biotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, Iran.
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources and International Innovation Center for Forest Chemicals and Materials, Nanjing Forestry University, Nanjing 210037, China.
Int J Biol Macromol. 2023 Apr 15;234:123636. doi: 10.1016/j.ijbiomac.2023.123636. Epub 2023 Feb 11.
A wide range of high-Z nanomaterials are fabricated to decrease radiation dose by sensitizing cells to irradiation through various mechanisms such as ROS generation enhancement. Alginate-coated silver sulfide nanoparticles (AgS@Alg) were synthesized and characterized by SEM, TEM, DLS, XRD, EPS, FT-IR, and UV-vis analysis techniques. Cytotoxicity of nanoparticles was tested against HFF-2, MCF-7, and 4 T1 cell lines for biocompatibility and radio enhancement ability evaluation, respectively. Moreover, the hemolysis assay demonstrated that the nanoparticles were biocompatible and nontoxic. In vitro intracellular ROS generation and calcein AM/PI co-staining unveiled cancerous cell death induction by nanoradiosensitizer, AgS@Alg. Further, histopathology results emphasized the tumor ablation capability of AgS@Alg. Silver anticancer properties were also recognized and combined with its radiosensitizing effect under X-ray irradiation.
人们制备了多种高Z值纳米材料，通过增强活性氧生成等各种机制使细胞对辐射敏感，从而降低辐射剂量。合成了海藻酸盐包覆的硫化银纳米颗粒（AgS@Alg），并通过扫描电子显微镜（SEM）、透射电子显微镜（TEM）、动态光散射（DLS）、X射线衍射（XRD）、能谱分析（EPS）、傅里叶变换红外光谱（FT-IR）和紫外可见光谱分析技术对其进行了表征。分别针对HFF-2、MCF-7和4T1细胞系测试了纳米颗粒的细胞毒性，以评估其生物相容性和放射增强能力。此外，溶血试验表明纳米颗粒具有生物相容性且无毒。体外细胞内活性氧生成和钙黄绿素AM/碘化丙啶共染色揭示了纳米放射增敏剂AgS@Alg可诱导癌细胞死亡。此外，组织病理学结果强调了AgS@Alg的肿瘤消融能力。还认识到银的抗癌特性，并将其与X射线照射下的放射增敏作用相结合。
