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鱼类红肌和猪肝的线粒体酰基辅酶A脱氢酶活性。

Mitochondrial Acyl-CoA dehydrogenase activity of fish red muscle and pig liver.

作者信息

Hamre K, van den Thillart G

机构信息

State University of Leiden, Dept. Animal Physiology, Gorlaeus Laboratories, The Netherlands.

出版信息

Comp Biochem Physiol B. 1987;88(1):19-22. doi: 10.1016/0305-0491(87)90071-x.

DOI:10.1016/0305-0491(87)90071-x
PMID:3677598
Abstract
  1. A method is described for the estimation of in vivo activity of mitochondrial Acyl-CoA dehydrogenase, without the addition of extra ETF (electron transferring flavoprotein). 2. Using Fumarase as a marker enzyme, the activity of Acyl-CoA dehydrogenase was calculated per gram wet weight. 3. The enzyme displays negative cooperativity in fish muscle, but positive cooperativity in pig liver. S0.5 values for the substrate octanoyl-CoA are between 3 and 5 microM.
摘要
  1. 本文描述了一种无需额外添加电子传递黄素蛋白(ETF)来估算线粒体酰基辅酶A脱氢酶体内活性的方法。2. 以延胡索酸酶作为标记酶,按每克湿重计算酰基辅酶A脱氢酶的活性。3. 该酶在鱼肌肉中表现出负协同性,但在猪肝中表现出正协同性。底物辛酰辅酶A的S0.5值在3至5微摩尔之间。

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1
Mitochondrial Acyl-CoA dehydrogenase activity of fish red muscle and pig liver.鱼类红肌和猪肝的线粒体酰基辅酶A脱氢酶活性。
Comp Biochem Physiol B. 1987;88(1):19-22. doi: 10.1016/0305-0491(87)90071-x.
2
Energetics of beta-oxidation. Reduction potentials of general fatty acyl-CoA dehydrogenase, electron transfer flavoprotein, and fatty acyl-CoA substrates.β-氧化的能量学。一般脂肪酰基辅酶A脱氢酶、电子传递黄素蛋白和脂肪酰基辅酶A底物的还原电位。
J Biol Chem. 1986 Jun 15;261(17):7733-41.
3
Crystal structures of medium-chain acyl-CoA dehydrogenase from pig liver mitochondria with and without substrate.猪肝线粒体中链酰基辅酶A脱氢酶在有底物和无底物情况下的晶体结构。
Proc Natl Acad Sci U S A. 1993 Aug 15;90(16):7523-7. doi: 10.1073/pnas.90.16.7523.
4
Purification and characterization of the 2-methyl branched-chain Acyl-CoA dehydrogenase, an enzyme involved in NADH-dependent enoyl-CoA reduction in anaerobic mitochondria of the nematode, Ascaris suum.猪蛔虫厌氧线粒体中参与依赖NADH的烯酰辅酶A还原反应的2-甲基支链酰基辅酶A脱氢酶的纯化与特性分析
J Biol Chem. 1985 Apr 25;260(8):4770-7.
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Studies on electron transfer from general acyl-CoA dehydrogenase to electron transfer flavoprotein.关于从一般酰基辅酶A脱氢酶到电子传递黄素蛋白的电子转移研究。
J Biol Chem. 1980 Apr 25;255(8):3591-5.
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Assay of acyl-CoA dehydrogenases in muscle and liver and identification of four new cases of medium-chain acyl-CoA dehydrogenase deficiency associated with systemic carnitine deficiency.肌肉和肝脏中酰基辅酶A脱氢酶的检测以及4例与全身性肉碱缺乏相关的新的中链酰基辅酶A脱氢酶缺乏症病例的鉴定。
Adv Neurol. 1988;48:231-7.
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Preferential cross-linking of the small subunit of the electron-transfer flavoprotein to general acyl-CoA dehydrogenase.电子传递黄素蛋白小亚基与通用酰基辅酶A脱氢酶的优先交联。
Biochem J. 1987 Apr 15;243(2):519-24. doi: 10.1042/bj2430519.
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Stabilization of the red semiquinone form of pig kidney general acyl-CoA dehydrogenase by acyl coenzyme A derivatives.酰基辅酶A衍生物对猪肾通用酰基辅酶A脱氢酶红色半醌形式的稳定作用。
Biochemistry. 1981 Aug 18;20(17):4965-70. doi: 10.1021/bi00520a024.
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Purification of electron transfer flavoprotein from pig liver mitochondria and its application to the diagnosis of deficiencies of acyl-CoA dehydrogenases in human fibroblasts.猪肝线粒体电子传递黄素蛋白的纯化及其在人成纤维细胞中酰基辅酶A脱氢酶缺乏症诊断中的应用。
Clin Chim Acta. 1992 Sep 15;210(1-2):75-91. doi: 10.1016/0009-8981(92)90047-t.
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Enzyme-activated inhibitors, alternate substrates, and a dead end inhibitor of the general acyl-CoA dehydrogenase.酶激活抑制剂、替代底物以及通用酰基辅酶A脱氢酶的一种终产物抑制剂。
J Biol Chem. 1980 Dec 10;255(23):11192-8.