牛血清血红素结合蛋白：来自单一动物的蛋白质特性

Bovine serum hemopexin: properties of the protein from a single animal.

Noiva R, Pete M J, Babin D R

Department of Biological Chemistry, Creighton University School of Medicine, Omaha, NE 68178.

Comp Biochem Physiol B. 1987;88(1):341-7. doi: 10.1016/0305-0491(87)90125-8.

Abstract

Hemopexin was isolated from bovine serum of a single animal in a yield of 0.5 mg/ml. 2. Bovine hemopexin was found to exist in two isoforms of mol. wt 68,000 and 65,000. 3. Treatment of hemopexin with glycopeptidase F yields a single band corresponding to a mol. wt of 51,000. 4. The protein binds heme on an equimolar ratio and shows a single component in reverse-phase high performance liquid chromatography. 5. The amino acid composition of bovine hemopexin compares with that of hemopexin isolated form other animals.

摘要

从一头牛的血清中分离出血红素结合蛋白，产量为0.5毫克/毫升。2. 发现牛血红蛋白结合蛋白以分子量为68,000和65,000的两种同工型存在。3. 用糖肽酶F处理血红蛋白结合蛋白产生一条对应分子量为51,000的条带。4. 该蛋白质以等摩尔比结合血红素，在反相高效液相色谱中显示单一成分。5. 牛血红蛋白结合蛋白的氨基酸组成与从其他动物分离的血红蛋白结合蛋白的氨基酸组成进行了比较。