免疫功能低下儿童呼吸道腺病毒载量的数字PCR检测及其临床相关性
Clinical Correlation of Adenoviral Load in the Respiratory Tract Measured by Digital PCR in Immunocompromised Children.
作者信息
Hijano Diego R, Ferrolino Jose A, Hidinger Jennifer, Brazelton Jessica N, Gu Zhengming, Suganda Sri, Glasgow Heather L, Allison Kim J, Peterson Megan, Dallas Ronald H, Maron Gabriela, Hayden Randall T
机构信息
Department of Infectious Diseases, St. Jude Children Research Hospital, Memphis, Tennessee, USA.
Department of Pathology, St. Jude Children Research Hospital, Memphis, Tennessee, USA.
出版信息
Open Forum Infect Dis. 2023 Jan 23;10(2):ofad030. doi: 10.1093/ofid/ofad030. eCollection 2023 Feb.
Abstract
Immunocompromised patients can have life-threatening adenoviral infection. Viral load in blood and stool is commonly used to guide antiviral therapy. We developed and evaluated a digital polymerase chain reaction assay to quantify human adenovirus in the respiratory tract and showed that higher peak load correlates with disseminated infection, mechanical ventilation, and death.
摘要
免疫功能低下的患者可能会发生危及生命的腺病毒感染。血液和粪便中的病毒载量通常用于指导抗病毒治疗。我们开发并评估了一种数字聚合酶链反应检测方法，用于定量呼吸道中的人腺病毒，并表明较高的峰值载量与播散性感染、机械通气和死亡相关。
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