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电针减轻糖尿病性神经病理性疼痛并下调背根神经节和脊髓背角中的p-PKC和TRPV1。

Electroacupuncture Alleviates Diabetic Neuropathic Pain and Downregulates p-PKC and TRPV1 in Dorsal Root Ganglions and Spinal Cord Dorsal Horn.

作者信息

Ma Yi-Qi, Hu Qun-Qi, Kang Yu Rong, Ma Li-Qian, Qu Si-Ying, Wang Han-Zhi, Zheng Yin-Mu, Li Si-Yi, Shao Xiao-Mei, Li Xiao-Yu, Hu Han-Tong, Jiang Yong-Liang, Fang Jian-Qiao, He Xiao-Fen

机构信息

Key Laboratory of Acupuncture and Neurology of Zhejiang Province, Department of Neurobiology and Acupuncture Research, The Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China.

出版信息

Evid Based Complement Alternat Med. 2023 Feb 3;2023:3333563. doi: 10.1155/2023/3333563. eCollection 2023.

DOI:10.1155/2023/3333563
PMID:36777630
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9918371/
Abstract

Diabetic neuropathic pain (DNP) is a common complication of diabetes. Streptozotocin (STZ)-induced changes of protein in dorsal root ganglion (DRG) and spinal cord dorsal horn (SCDH) are critical for DNP genesis. However, which proteins change remains elusive. Here, the DNP model was established by a single intraperitoneal injection of STZ, accompanied by increased fasting blood glucose (FBG), decreased body weight (BW), and decreased paw withdrawal latency (PWL). Proteins change in L4-L6 DRGs and SCDH of rats were detected. Western blot and immunofluorescence results showed that expression levels of phosphorylated protein kinase C (p-PKC), transient receptor potential vanilloid-1 (TRPV1), Substance P (SP) and calcitonin gene-related peptide (CGRP) in the DRG and the SCDH of rats were increased after STZ injection. A preliminary study from our previous study showed that 2 Hz electroacupuncture (EA) effectively alleviates DNP. However, the analgesic mechanism of EA needs further elucidation. Here, EA at the bilateral Zusanli (ST36) and KunLun (BL60) acupoints was applied for one week, and to investigate the effect on DNP. EA reversed thermal hyperalgesia in DNP rats and downregulated the expression of p-PKC, TRPV1, SP, and CGRP in DRG and SCDH.

摘要

糖尿病性神经病理性疼痛（DNP）是糖尿病的常见并发症。链脲佐菌素（STZ）诱导的背根神经节（DRG）和脊髓背角（SCDH）中蛋白质的变化对DNP的发生至关重要。然而，哪些蛋白质发生了变化仍不清楚。在此，通过单次腹腔注射STZ建立DNP模型，同时伴有空腹血糖（FBG）升高、体重（BW）下降和爪部退缩潜伏期（PWL）缩短。检测大鼠L4-L6 DRG和SCDH中的蛋白质变化。蛋白质免疫印迹和免疫荧光结果显示，注射STZ后，大鼠DRG和SCDH中磷酸化蛋白激酶C（p-PKC）、瞬时受体电位香草酸受体1（TRPV1）、P物质（SP）和降钙素基因相关肽（CGRP）的表达水平升高。我们之前研究的初步结果表明，2Hz电针（EA）可有效缓解DNP。然而，EA的镇痛机制需要进一步阐明。在此，对双侧足三里（ST36）和昆仑（BL60）穴位进行一周的电针治疗，以研究其对DNP的影响。电针逆转了DNP大鼠的热痛觉过敏，并下调了DRG和SCDH中p-PKC、TRPV1、SP和CGRP的表达。

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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/781b/9918371/7d4283d41537/ECAM2023-3333563.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/781b/9918371/4e040e045595/ECAM2023-3333563.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/781b/9918371/588a70a06b42/ECAM2023-3333563.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/781b/9918371/43f3b24e5e1d/ECAM2023-3333563.009.jpg

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