[电针对膝骨关节炎大鼠脊髓背角和背根神经节疼痛行为及疼痛相关因素的影响]

[Effects of electroacupuncture on pain behavior and pain-related factors in spinal cord dorsal horn and dorsal root ganglia of rats with knee osteoarthritis].

作者信息

Yang Kun-Wu, Yuan Pu-Wei, Dong Bo, Fan Jing-Jie, Wang Ying-Ying, Liu Hui, Chai Xin, Zheng Jie

机构信息

Institution of Acupuncture-moxibustion and Massage, Shaanxi University of Chinese Medicine, Xianyang 712046, Shaanxi Province, China; Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700.

Affiliated Hospital of Shaanxi University of Chinese Medicine, Xianyang 712000, Shaanxi Province.

出版信息

Zhen Ci Yan Jiu. 2020 Oct 25;45(10):818-22. doi: 10.13702/j.1000-0607.200540.

DOI:10.13702/j.1000-0607.200540

PMID:33788448

Abstract

OBJECTIVE

To observe the effect of electroacupuncture(EA) on the pain behavior and prostaglandin E2 (PGE2), calcitonin gene-related peptide (CGRP) and substance P (SP) in the spinal cord dorsal horn and dorsal root ganglia (DRG) of rats with knee osteoarthritis (KOA), so as to explore the mechanisms of EA underlying improvement of chronic pain in KOA rats.

METHODS

Thirty-two female SD rats were randomly divided into blank group, control group, model group and EA group, with 8 rats in each group. Rats in the control group were injected with 50 μL of 0.9% sodium chloride solution into the left knee joint cavity, and rats in the model and EA groups were injected with 50 μL of Monosodium iodoacetate in the left knee joint. EA(2 Hz/100 Hz, ＜2 mA) was applied to left "Yanglingquan"(GB34) and "Neixiyan" (EX-LE4) for 15 min, once daily, 5 days a course with a total of 2 courses. Paw withdrawal latency (PWL) and mechanical pain threshold (PWT) were tested by Plantar Test and Von Frey, separately. After the last pain test, the contents of PGE2, CGRP and SP in the left lumbar (L) 3-L5 DRG and L3-L5 spinal dorsal horn were detected by ELISA.

RESULTS

Compared with the blank group, the PWL and PWT of the rats in the model group were significantly decreased (<0.01), and the contents of PGE2, CGRP and SP in the DRG and spinal dorsal horn were significantly increased (<0.01, <0.05). There were no statistically significant differences in PWL, PWT, contents of PGE2, CGRP and SP in DRG and spinal dorsal horn between the blank group and the control group (>0.05). Compared with the model group, the PWL and PWT of rats in the EA group were significantly increased (<0.01), and the contents of PGE2, CGRP and SP in the DRG and spinal dorsal horn were significantly decreased (<0.01, <0.05).

CONCLUSION

EA of GB34 and EX-LE4 can reduce the levels of pain-related factors PGE2, CGRP and SP in the DRG and spinal dorsal horn, thereby relieving spinal hyperalgesia in rats with KOA.

摘要

目的

观察电针（EA）对膝骨关节炎（KOA）大鼠疼痛行为及脊髓背角和背根神经节（DRG）中前列腺素E2（PGE2）、降钙素基因相关肽（CGRP）和P物质（SP）的影响，以探讨EA改善KOA大鼠慢性疼痛的机制。

方法

32只雌性SD大鼠随机分为空白组、对照组、模型组和电针组，每组8只。对照组大鼠左膝关节腔内注射50 μL 0.9%氯化钠溶液，模型组和电针组大鼠左膝关节腔内注射50 μL碘乙酸钠。将电针（2 Hz/100 Hz，＜2 mA）施加于左侧“阳陵泉”（GB34）和“内膝眼”（EX-LE4）15分钟，每日1次，5天为1个疗程，共2个疗程。分别采用足底试验和von Frey法检测爪部撤离潜伏期（PWL）和机械性疼痛阈值（PWT）。末次疼痛测试后，采用酶联免疫吸附测定（ELISA）法检测左侧腰（L）3-L5 DRG和L3-L5脊髓背角中PGE2、CGRP和SP的含量。

结果

与空白组比较，模型组大鼠的PWL和PWT显著降低（<0.01），DRG和脊髓背角中PGE2、CGRP和SP的含量显著升高（<0.01，<0.05）。空白组与对照组大鼠的PWL、PWT、DRG和脊髓背角中PGE2、CGRP和SP的含量比较，差异无统计学意义（>0.05）。与模型组比较，电针组大鼠的PWL和PWT显著升高（<0.01），DRG和脊髓背角中PGE2、CGRP和SP的含量显著降低（<0.01，<0.05）。