Department of Respiratory Medicine, Zhejiang Jinhua Guangfu Cancer Hospital, Jinhua City, Zhejiang Province, 321000, China.
Department of Laboratory, Jinhua Wenrong Hospital, Jinhua City, Zhejiang Province, 322118, China.
Pharmacogenomics. 2023 Feb;24(3):163-172. doi: 10.2217/pgs-2022-0121. Epub 2023 Feb 13.
To investigate the influence of on paclitaxel (PTX) resistance in lung adenocarcinoma (LUAD). LncRNA expression and functional enrichment analyses were processed via bioinformatics methods. expression was detected via quantitative real-time PCR. Cell counting kit-8, colony formation, flow cytometry and comet assays were manipulated to measure cell proliferation, apoptosis, cell cycle and DNA damage repair, respectively. Western blot was used to assess DNA damage-related protein expression. was in a high expression status in LUAD, and could promote LUAD cell proliferation and PTX resistance, while suppressing cell apoptosis. DNA damage repairing ability was also modulated by the change of expression. LncRNA promotes DNA damage repair to enhance PTX resistance in LUAD.
为了研究lncRNA 在肺腺癌（LUAD）中对紫杉醇（PTX）耐药性的影响。我们通过生物信息学方法进行了 lncRNA 表达和功能富集分析。通过实时定量 PCR 检测 表达。细胞计数试剂盒-8、集落形成、流式细胞术和彗星试验分别用于测量细胞增殖、细胞凋亡、细胞周期和 DNA 损伤修复。Western blot 用于评估与 DNA 损伤相关的蛋白表达。lncRNA 在 LUAD 中呈高表达状态，能够促进 LUAD 细胞增殖和 PTX 耐药性，同时抑制细胞凋亡。表达变化也调节了 DNA 损伤修复能力。lncRNA 通过促进 DNA 损伤修复来增强 LUAD 中的 PTX 耐药性。
