基于 SNP 标记的多重测序基因分型 (GMS)

Genotyping by Multiplexed Sequencing (GMS) Using SNP Markers.

机构信息

USDA-ARS Wheat Health, Genetics and Quality Research Unit, Pullman, WA, USA.

Department of Plant Pathology, Washington State University, Pullman, WA, USA.

出版信息

Methods Mol Biol. 2023;2638:9-21. doi: 10.1007/978-1-0716-3024-2_2.

DOI:10.1007/978-1-0716-3024-2_2

PMID:36781632

Abstract

SNP-based genotyping has become the most effective approach to generate target-specific data for use in genetic studies. In this chapter, we will describe a high-throughput genotyping method that multiplexes hundreds to thousands of SNP markers in a two-step PCR protocol that can be customized to fit the specific needs of a study.

摘要

基于 SNP 的基因分型已成为生成用于遗传研究的目标特异性数据的最有效方法。在本章中，我们将描述一种高通量基因分型方法，该方法通过两步 PCR 方案对数百到数千个 SNP 标记进行多重化，该方案可根据研究的具体需求进行定制。