Mikazuki K, Takahashi K, Nanba K, Hayashi Y
Aburahi Laboratories, Shionogi and Co., Ltd., Shiga, Japan.
Jikken Dobutsu. 1987 Jul;36(3):229-37.
Bacteria from the subcutaneous abscesses which appeared in a laboratory colony of DS mouse since October of 1977 were identified as Pasteurella pneumotropica by various biological examinations. The abscess formation was limited to multiparous female mice over 100 day-age, but virgin females were free from the disease. The MIC of various antibacterial substances showed that potassium tellurite, kanamycin and bacitracin were effective to isolate the organism selectively from various infection sites harboring many other species of bacteria. A novel NKBT medium was prepared by adding these antibacterial substances to the heart infusion agar (HIA) supplemented by 10% Fildes digested blood. A fluid culture medium, TGN broth was prepared for multiplication of the organism by adding 10% Fildes digested blood and potassium tellurite to GN broth. To isolate the organism from the pharyngo-larynx a direct application of mucus wiped off the infection site onto the culture medium was sufficient, but pre-multiplication in the TGN broth was required for isolation of the organism from gut contents before inoculation onto the NKBT medium. The pre-cultivation in the TGN broth vastly improved the recovery of the organism especially from feces. Thereby we could easily detect the latent infection of this bacterium without sacrificing animals.
自1977年10月起，在一个DS小鼠实验种群中出现了皮下脓肿，通过各种生物学检查，从这些脓肿中分离出的细菌被鉴定为嗜肺巴斯德杆菌。脓肿形成仅限于日龄超过100天的经产雌性小鼠，而处女雌鼠则未感染该病。各种抗菌物质的最低抑菌浓度表明，亚碲酸钾、卡那霉素和杆菌肽可有效从含有许多其他细菌种类的各种感染部位选择性分离该菌。通过将这些抗菌物质添加到补充有10%菲尔德消化血的心脏浸液琼脂（HIA）中，制备了一种新型的NKBT培养基。通过向GN肉汤中添加10%菲尔德消化血和亚碲酸钾，制备了一种液体培养基TGN肉汤，用于该菌的增殖。为了从咽喉部分离该菌，将从感染部位擦拭的黏液直接涂抹在培养基上就足够了，但在接种到NKBT培养基之前，需要在TGN肉汤中进行预增殖以从肠道内容物中分离该菌。在TGN肉汤中进行预培养极大地提高了该菌的回收率，尤其是从粪便中的回收率。由此，我们可以在不牺牲动物的情况下轻松检测到这种细菌的潜伏感染。
