Differential Effects of Virulence-Associated Proteins on Macrophages and Artificial Lipid Membranes.

irulence-ssociated rotein (VapA) of Rhodococcus equi is a pathogenicity factor required for the multiplication of virulent strains within spacious macrophage vacuoles. The production of VapA is characteristic for isolates from pneumonic foals. VapB and VapN proteins in isolates from infected pig (VapB) and cattle (VapN) have amino acid sequences very similar to VapA and consequently have been assumed to be its functional correlates. Using model membrane experiments, phagosome pH acidification analysis, lysosome size measurements, protein partitioning, and degradation assays, we provide support for the view that VapA and VapN promote intracellular multiplication of by neutralizing the pH of the -containing vacuole. VapB does not neutralize vacuole pH, is not as membrane active as VapA, and does not support intracellular multiplication. This study also shows that the size of the sometimes enormous -containing vacuoles or the partitioning of purified Vaps into organic phases are not features that have predictive value for virulence of , whereas the ability of Vaps to increase phagosome pH is coupled to virulence. Rhodococcus equi is a major cause of life-threatening pneumonia in foals and occasionally in immunocompromised persons. Virulence-associated protein A (VapA) promotes multiplication in lung macrophages, which are the major host cells during foal infection. In this study, we compare cellular, biochemical, and biophysical phenotypes associated with VapA to those of VapB (typically produced by isolates from pigs) or VapN (isolates from cattle). Our data support the hypothesis that only some Vaps support multiplication in macrophages by pH neutralization of the phagosomes that inhabit.