胆固醇依赖性细胞溶素：悬而未决的问题。
Cholesterol-dependent cytolysins: The outstanding questions.
机构信息
Department of Biochemistry and Pharmacology, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Parkville, Victoria, Australia.
ARC Centre for Cryo-electron Microscopy of Membrane Proteins, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Parkville, Victoria, Australia.
出版信息
IUBMB Life. 2022 Dec;74(12):1169-1179. doi: 10.1002/iub.2661. Epub 2022 Jul 14.
Abstract
The cholesterol-dependent cytolysins (CDCs) are a major family of bacterial pore-forming proteins secreted as virulence factors by Gram-positive bacterial species. CDCs are produced as soluble, monomeric proteins that bind specifically to cholesterol-rich membranes, where they oligomerize into ring-shaped pores of more than 30 monomers. Understanding the details of the steps the toxin undergoes in converting from monomer to a membrane-spanning pore is a continuing challenge. In this review we summarize what we know about CDCs and highlight the remaining outstanding questions that require answers to obtain a complete picture of how these toxins kill cells.
摘要
胆固醇依赖性细胞溶素（CDCs）是一大类细菌形成孔的蛋白家族，作为革兰氏阳性细菌的毒力因子被分泌出来。CDCs 以可溶性单体蛋白的形式产生，这些蛋白特异性地结合富含胆固醇的膜，在那里它们寡聚成超过 30 个单体的环形孔。了解毒素从单体转化为跨膜孔的过程中的细节一直是一个挑战。在这篇综述中，我们总结了我们对 CDCs 的了解，并强调了仍需要回答的悬而未决的问题，以获得这些毒素如何杀死细胞的完整图景。
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