Thompson M A, Hawkins J W, Piatigorsky J
Laboratory of Molecular and Developmental Biology, National Eye Institute, Bethesda, MD 20892.
Gene. 1987;56(2-3):173-84. doi: 10.1016/0378-1119(87)90135-1.
The chicken alpha A-crystallin gene and 2.6 kb of its 5' flanking sequence have been isolated and characterized by electron microscopy and sequencing. The structural gene is 4.5 kb long and contains two introns, each approx. 1 kb in length. The first intron divides codons 63 and 64, and the second intron divides codons 104 and 105, as in rodents. There is little indication that the insert exon of rodents (an alternatively spliced sequence) is present in complete form in the chicken alpha A-crystallin gene; small stretches of similarity to this sequence were found throughout the gene. The 5' flanking sequence of the chicken alpha A-crystallin gene shows considerable sequence similarity with other mammalian alpha B-crystallin genes. In addition, one consensus sequence (GCAGCATGCCCTCCTAG) present in the 5' flanking region of the chicken alpha A-crystallin gene was found in the 5' flanking region of most reported crystallin genes.
鸡αA-晶状体蛋白基因及其5'侧翼序列的2.6 kb片段已通过电子显微镜和测序进行了分离和表征。结构基因长4.5 kb，包含两个内含子，每个内含子长度约为1 kb。与啮齿动物一样，第一个内含子分隔密码子63和64，第二个内含子分隔密码子104和105。几乎没有迹象表明啮齿动物的插入外显子（一个选择性剪接序列）以完整形式存在于鸡αA-晶状体蛋白基因中；在整个基因中发现了与该序列的小片段相似性。鸡αA-晶状体蛋白基因的5'侧翼序列与其他哺乳动物αB-晶状体蛋白基因显示出相当大的序列相似性。此外，在大多数已报道的晶状体蛋白基因的5'侧翼区域中发现了鸡αA-晶状体蛋白基因5'侧翼区域中存在的一个共有序列（GCAGCATGCCCTCCTAG）。
