植物前体mRNA在动物系统中的剪接，反之亦然。

Splicing of plant pre-mRNAs in animal systems and vice versa.

作者信息

van Santen V L, Spritz R A

机构信息

Laboratory of Genetics, University of Wisconsin, Madison 53706.

出版信息

Gene. 1987;56(2-3):253-65. doi: 10.1016/0378-1119(87)90142-9.

DOI:10.1016/0378-1119(87)90142-9

PMID:3678838

Abstract

To investigate similarities and differences of pre-mRNA splicing between higher plants and animals, we tested whether pre-mRNAs of higher plants can be accurately spliced in animal systems, and whether an animal pre-mRNA can be spliced in plant cells. Pre-mRNAs of the maize bronze locus and oat phytochrome type 3 are accurately spliced with moderate efficiency in a human (HeLa cell) nuclear extract. The first intervening sequence (IVS1) of bean phaseolin pre-mRNA is not excised in the human nuclear extract, but is removed (although at low efficiency) in intact monkey cells. However, the IVS1 of human alpha-globin pre-mRNA is not removed in tobacco cells. Our data suggest that the mechanisms of pre-mRNA splicing are similar, but not identical, in plants and animals.

摘要

为了研究高等植物和动物前体mRNA剪接的异同，我们测试了高等植物的前体mRNA是否能在动物系统中准确剪接，以及动物前体mRNA是否能在植物细胞中剪接。玉米青铜位点和燕麦3型光敏色素的前体mRNA在人（HeLa细胞）核提取物中能以中等效率准确剪接。菜豆球蛋白前体mRNA的第一个内含序列（IVS1）在人核提取物中未被切除，但在完整的猴细胞中被切除（尽管效率较低）。然而，人α-珠蛋白前体mRNA的IVS1在烟草细胞中未被切除。我们的数据表明，植物和动物中前体mRNA剪接机制相似但不完全相同。