人皮肤成纤维细胞体外老化过程中通过磷酸戊糖途径的葡萄糖通量及NADP(H)水平

Glucose flux through the hexose monophosphate shunt and NADP(H) levels during in vitro ageing of human skin fibroblasts.

作者信息

Jongkind J F, Verkerk A, Poot M

机构信息

Department of Cell Biology and Genetics, Erasmus University, Rotterdam, The Netherlands.

出版信息

Gerontology. 1987;33(5):281-6. doi: 10.1159/000212891.

DOI:10.1159/000212891

PMID:3678843

Abstract

In cultured human skin fibroblasts the glucose flux through the hexose monophosphate shunt (HMS) amounts to 4% of the glucose flux through the glycolytic pathway. Upon in vitro ageing the rate of glucose utilization through the HMS is decreased more than 50%. This decrease in HMS was not caused by a limiting enzymatic capacity since glucose utilization through the HMS could be raised at least 30-fold in both 'young' and 'aged' fibroblasts upon stimulation with phenazine methosulphate. This effect of in vitro ageing upon glucose metabolism was also not due to differences in proliferation rate between 'young' and 'aged' human fibroblasts, since there was no difference in glucose utilization between proliferating and growth-inhibited (confluently cultured) fibroblasts. The NADPH/NADP ratio was found to be decreased by 12% in 'aged' cells.

摘要

在培养的人皮肤成纤维细胞中，通过磷酸己糖途径（HMS）的葡萄糖通量占通过糖酵解途径的葡萄糖通量的4%。体外老化后，通过HMS的葡萄糖利用率下降超过50%。HMS的这种下降不是由酶促能力受限引起的，因为在用吩嗪硫酸甲酯刺激后，“年轻”和“衰老”成纤维细胞中通过HMS的葡萄糖利用率都可以提高至少30倍。体外老化对葡萄糖代谢的这种影响也不是由于“年轻”和“衰老”人成纤维细胞增殖速率的差异，因为增殖的和成纤维细胞（汇合培养）之间的葡萄糖利用率没有差异。发现“衰老”细胞中的NADPH/NADP比值下降了12%。