Institute of Forensic Medicine, Forensic Toxicology and Chemistry, University of Bern, Murtenstrasse 26, 3008 Bern, Switzerland.
Institute of Technology Management, University of St. Gallen, Dufourstrasse 40a, 9000 St. Gallen, Switzerland.
J Anal Toxicol. 2023 Apr 14;47(4):379-384. doi: 10.1093/jat/bkad009.
Direct alcohol biomarkers are of growing interest for the assessment of alcohol consumption, with particular interest in phosphatidylethanol (PEth) in recent years. PEth is only formed when alcohol is present in the body. However, there is no statement about how much the PEth concentration increases after single moderate alcohol consumption. This study was conducted to determine the increase in PEth concentrations after a single drinking event. Additionally, a new volumetric sampling device (volumetric dried blood spot cards (DBSV)) was evaluated, which was designed to simplify further sampling processes and to allow for easy self-sampling. Dried blood samples from 31 volunteers were collected before and after single alcohol consumption with a mean maximum breath alcohol concentration of 0.4 mg/L (range: 0.30-0.55 mg/L). PEth concentrations were determined after automated extraction by liquid chromatography--tandem mass spectrometry. PEth 16:0/18:1 and PEth 16:0/18:2 concentrations increased to an average of 45 ng/mL each in patients starting below 20 ng/mL (range: 25.0-57.0 ng/mL for PEth 16:0/18:1; range 26.8-62.3 ng/mL for PEth 16:0/18:2). PEth concentrations in patients starting above 20 ng/mL increased by a mean of 30 ng/mL (range: 6.2-71.3 ng/mL for PEth 16:0/18:1; range 8.8-65.3 ng/mL for PEth 16:0/18:2). In addition, the comparison of the new sampling device DBSV with a standard filter paper card (with volumetrically applied 20 µL of blood samples) yielded a close agreement for the determined PEth concentrations in 24 forensic samples and three external controls. Therefore, the sampling device DBSV proved to be suitable for the determination of PEth concentrations in blood.
直接的酒精生物标志物对于评估酒精摄入量越来越受到关注，近年来，特别是对磷脂酰乙醇（PEth）的研究兴趣浓厚。只有当体内存在酒精时，才会形成 PEth。然而，对于单次适度饮酒后 PEth 浓度增加多少，目前尚无定论。本研究旨在确定单次饮酒事件后 PEth 浓度的增加。此外，还评估了一种新的容积采样装置（容积式干血斑卡（DBSV）），旨在简化进一步的采样过程，并允许轻松进行自我采样。在平均最大呼气酒精浓度为 0.4mg/L（范围：0.30-0.55mg/L）的情况下，31 名志愿者在单次饮酒前后采集了干血样。通过液相色谱-串联质谱法自动提取后测定 PEth 浓度。PEth 16:0/18:1 和 PEth 16:0/18:2 浓度在起始浓度低于 20ng/mL（PEth 16:0/18:1 范围为 25.0-57.0ng/mL；PEth 16:0/18:2 范围为 26.8-62.3ng/mL）的患者中平均增加到 45ng/mL 每个。起始浓度高于 20ng/mL 的患者平均增加 30ng/mL（PEth 16:0/18:1 范围为 6.2-71.3ng/mL；PEth 16:0/18:2 范围为 8.8-65.3ng/mL）。此外，新的采样装置 DBSV 与标准滤纸卡（体积应用 20μL 血样）的比较表明，在 24 个法医样本和 3 个外部对照中，测定的 PEth 浓度非常吻合。因此，采样装置 DBSV 被证明适用于血液中 PEth 浓度的测定。
