Florey Institute of Neuroscience and Mental Health, Parkville, VIC 3052, Australia; Florey Department of Neuroscience and Mental Health, University of Melbourne, Parkville, VIC 3052, Australia.
Florey Institute of Neuroscience and Mental Health, Parkville, VIC 3052, Australia; Life Sciences Center, Vilnius University, 10257 Vilnius, Lithuania.
Cell Rep. 2023 Feb 28;42(2):112122. doi: 10.1016/j.celrep.2023.112122. Epub 2023 Feb 14.
Exposure to cocaine leads to robust changes in the structure and function of neurons within the mesocorticolimbic pathway. However, little is known about how cocaine influences the processing of information within the sensory cortex. We address this by using patch-clamp and juxtacellular voltage recordings and two-photon Ca imaging in vivo to investigate the influence of acute cocaine exposure on layer 2/3 (L2/3) pyramidal neurons within the primary somatosensory cortex (S1). Here, cocaine dampens membrane potential state transitions and decreases spontaneous somatic action potentials and Ca transients. In contrast to the uniform decrease in background spontaneous activity, cocaine has a heterogeneous influence on sensory encoding, increasing tactile-evoked responses in dendrites that do not typically encode sensory information and decreasing responses in those dendrites that are more reliable sensory encoders. Combined, these findings suggest that cocaine acts as a filter that suppresses background noise to selectively modulate incoming sensory information.
可卡因暴露会导致中脑边缘多巴胺能通路中神经元的结构和功能发生剧烈变化。然而，目前对于可卡因如何影响感觉皮层内的信息处理知之甚少。我们通过使用膜片钳和细胞外电压记录以及体内双光子 Ca 成像来解决这个问题，研究急性可卡因暴露对初级体感皮层（S1）中第 2/3 层（L2/3）锥体神经元的影响。在这里，可卡因抑制了膜电位状态转变，并减少了自发性体动作电位和 Ca 瞬变。与背景自发性活动的均匀减少相反，可卡因对感觉编码有不均匀的影响，增加了通常不编码感觉信息的树突中的触觉诱发电位反应，并降低了那些更可靠的感觉编码树突的反应。综合这些发现表明，可卡因作为一种滤波器，抑制背景噪声，选择性地调节传入的感觉信息。
