Li Guomeng, Liu Peng, Zhao Jihan, Su Liangyinan, Zhao Mengyu, Jiang Zhengjie, Zhao Yang, Yang Xiping
State Key Laboratory of Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Key Laboratory of Sugarcane Biology, Guangxi University, Nanning, China.
Key Laboratory of Crop Cultivation and Tillage, College of Agriculture, Guangxi University, Nanning, China.
Front Microbiol. 2023 Jan 30;14:1088532. doi: 10.3389/fmicb.2023.1088532. eCollection 2023.
Traditional chemical control methods pose a damaging effect on farmland ecology, and their long-term use has led to the development of pest resistance.
Here, we analyzed the correlations and differences in the microbiome present in the plant and soil of sugarcane cultivars exhibiting different insect resistance to investigate the role played by microbiome in crop insect resistance. We evaluated the microbiome of stems, topsoil, rhizosphere soil, and striped borers obtained from infested stems, as well as soil chemical parameters.
Results showed that microbiome diversity was higher in stems of insect-resistant plants, and contrast, lower in the soil of resistant plants, with fungi being more pronounced than bacteria. The microbiome in plant stems was almost entirely derived from the soil. The microbiome of insect-susceptible plants and surrounding soil tended to change towards that of insect-resistant plants after insect damage. Insects' microbiome was mainly derived from plant stems and partly from the soil. Available potassium showed an extremely significant correlation with soil microbiome. This study validated the role played by the microbiome ecology of plant-soil-insect system in insect resistance and provided a pre-theoretical basis for crop resistance control.
传统化学防治方法对农田生态造成破坏，其长期使用导致害虫产生抗性。
在此，我们分析了对不同昆虫具有抗性的甘蔗品种的植物和土壤中存在的微生物群落的相关性和差异，以研究微生物群落在作物抗虫性中所起的作用。我们评估了从受侵染茎中获得的茎、表土、根际土壤和条螟的微生物群落，以及土壤化学参数。
结果表明，抗虫植物茎中的微生物群落多样性较高，相比之下，抗性植物土壤中的微生物群落多样性较低，真菌比细菌更明显。植物茎中的微生物群落几乎完全来自土壤。感虫植物及其周围土壤的微生物群落在受到虫害后倾向于向抗虫植物的微生物群落转变。昆虫的微生物群落主要来自植物茎，部分来自土壤。有效钾与土壤微生物群落呈极显著相关。本研究验证了植物-土壤-昆虫系统的微生物群落生态在抗虫性中所起的作用，并为作物抗性控制提供了理论前期基础。
