Bumunang Emmanuel W, McAllister Tim A, Polo Rodrigo Ortega, Ateba Collins N, Stanford Kim, Schlechte Jared, Walker Matthew, MacLean Kellie, Niu Yan D
Department of Ecosystem and Public Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, Canada.
Agriculture and Agri-Food Canada, Lethbridge Research and Development Centre, Lethbridge, Canada.
Phage (New Rochelle). 2022 Dec 1;3(4):221-230. doi: 10.1089/phage.2022.0003. Epub 2022 Dec 19.
Non-O157 Shiga toxigenic (STEC) are one of the most important food and waterborne pathogens worldwide. Although bacteriophages (phages) have been used for the biocontrol of these pathogens, a comprehensive understanding of the genetic characteristics and lifestyle of potentially effective candidate phages is lacking.
In this study, 10 non-O157-infecting phages previously isolated from feedlot cattle and dairy farms in the North-West province of South Africa were sequenced, and their genomes were analyzed.
Comparative genomics and proteomics revealed that the phages were closely related to other -infecting , , , , and from the National Center for Biotechnology Information GenBank database. Phages lacked integrases associated with a lysogenic cycle and genes associated with antibiotic resistance and Shiga toxins.
Comparative genomic analysis identified a diversity of unique non-O157-infecting phages, which could be used to mitigate the abundance of various non-O157 STEC serogroups without safety concerns.
非O157产志贺毒素大肠杆菌(STEC)是全球最重要的食源性病原体和水源性病原体之一。尽管噬菌体已被用于对这些病原体进行生物防治,但对潜在有效候选噬菌体的遗传特征和生活方式仍缺乏全面了解。
在本研究中,对之前从南非西北省的饲养场牛和奶牛场分离出的10种非O157感染性噬菌体进行了测序,并对其基因组进行了分析。
比较基因组学和蛋白质组学显示,这些噬菌体与美国国立生物技术信息中心基因库数据库中的其他感染、、、、和的噬菌体密切相关。噬菌体缺乏与溶原周期相关的整合酶以及与抗生素抗性和志贺毒素相关的基因。
比较基因组分析鉴定出多种独特的非O157感染性噬菌体,可用于减少各种非O157 STEC血清群的数量,且不存在安全问题。