ELISA Essentials: Surfaces, Antibodies, Enzymes, and Substrates.

The enzyme-linked immunosorbent assay is a powerful analytical tool for the assessment of the kind and quantity of specific analytes found within a biological sample. It is based upon the exceptional specificity of antibody recognition of its cognate antigen and the power of enzyme-mediated signal amplification for sensitivity. However, development of the assay is not without challenges. Here, we describe the essential components and features necessary to successfully prepare and carry out the ELISA format.