ELISA 法检测抗体特异性和敏感性。
Screening for Antibody Specificity and Sensitivity with ELISA.
机构信息
Omega Biologicals, Inc., Bozeman, MT, USA.
出版信息
Methods Mol Biol. 2023;2612:45-58. doi: 10.1007/978-1-0716-2903-1_4.
Monoclonal antibodies (MAbs) can be used to detect and quantify protein biomarker antigens (Ag). Systematic screening with an enzyme-linked immunosorbent assay (Butler, J Immunoass, 21(2-3):165-209, 2000) [1] can be used to identify matched Ab-Ag pairs. A method is described for identifying MAbs that recognize cardiac biomarker creatine kinase isoform MB. Cross-reactivity with skeletal muscle biomarker creatine kinase isoform MM and brain biomarker creatine kinase isoform BB is also examined.
单克隆抗体 (MAbs) 可用于检测和定量蛋白质生物标志物抗原 (Ag)。酶联免疫吸附测定 (Butler, J Immunoass, 21(2-3):165-209, 2000) [1] 的系统筛选可用于识别匹配的 Ab-Ag 对。本文描述了一种识别识别心脏生物标志物肌酸激酶同工酶 MB 的单克隆抗体的方法。还研究了与骨骼肌生物标志物肌酸激酶同工酶 MM 和脑生物标志物肌酸激酶同工酶 BB 的交叉反应性。
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