Hou Dongmin, Chen Huibao, Jia Ting, Zhang Lin, Gao Wenrong, Chen Simeng, Zhu Wanlong
Key Laboratory of Ecological Adaptive Evolution and Conservation on Animals-Plants in Southwest Mountain Ecosystem of Yunnan, School of Life Sciences, Yunnan Normal University, Kunming, 650500, China.
Yunnan University of Business Management, Kunming, 650106, China.
J Therm Biol. 2023 Feb;112:103406. doi: 10.1016/j.jtherbio.2022.103406. Epub 2022 Dec 9.
In order to investigate the influence of gradient cooling acclimation on body mass regulation in tree shrews (Tupaia belangeri), white adipose tissue (WAT) and brown adipose tissue (BAT) in T. belangeri between the control group and gradient cooling acclimation group on day 56 were collected, body mass, food intake, thermogenic capacity, differential metabolites, and related metabolic pathways in WAT and BAT were measured, the changes of differential metabolites were analyzed by non-targeted metabolomics method based on liquid chromatography-mass spectrometry. The results shown that gradient cooling acclimation significantly increased body mass, food intake, resting metabolic rate (RMR), non-shivering thermogenesis (NST), and masses of WAT and BAT. 23 significant differential metabolites in WAT between the gradient cooling acclimation group and the control group, of which the relative contents of 13 differential metabolites were up-regulated and 10 differential metabolites were down-regulated. 27 significant differential metabolites in BAT, of which 18 differential metabolites decreased and 9 differential metabolites increased. 15 differential metabolic pathways in WAT, 8 differential metabolic pathways in BAT, and 4 differential metabolic pathways involved in both WAT and BAT, including Purine metabolism, Pyrimidine metabolism, Glycerol phosphate metabolism, Arginine and proline metabolism, respectively. All of the above results suggested that T. belangeri could use different metabolites of adipose tissue to withstand low temperature environments and enhance their survival.
为了研究梯度冷却驯化对树鼩（Tupaia belangeri）体重调节的影响，在第56天收集了对照组和梯度冷却驯化组树鼩的白色脂肪组织（WAT）和棕色脂肪组织（BAT），测量了WAT和BAT中的体重、食物摄入量、产热能力、差异代谢物及相关代谢途径，采用基于液相色谱-质谱联用的非靶向代谢组学方法分析差异代谢物的变化。结果显示，梯度冷却驯化显著增加了体重、食物摄入量、静息代谢率（RMR）、非颤抖性产热（NST）以及WAT和BAT的质量。梯度冷却驯化组与对照组相比，WAT中有23种显著差异代谢物，其中13种差异代谢物的相对含量上调，10种差异代谢物下调。BAT中有27种显著差异代谢物，其中18种差异代谢物减少，9种差异代谢物增加。WAT中有15条差异代谢途径，BAT中有8条差异代谢途径，WAT和BAT共有的差异代谢途径有4条，分别为嘌呤代谢、嘧啶代谢、甘油磷酸代谢、精氨酸和脯氨酸代谢。上述所有结果表明，树鼩可利用脂肪组织中的不同代谢物来抵御低温环境并提高其生存能力。
