Qi Ruohan, Pei Jiahe, Zhou Quan, Hao Keyu, Tian Yi, Ren Lili, Luo Youqing
Beijing Key Laboratory for Forest Pest Control, Beijing Forestry University, Beijing 100083, China.
Int J Mol Sci. 2024 Nov 26;25(23):12716. doi: 10.3390/ijms252312716.
L. can attract adult Asian longhorned beetle (ALB), (Motschulsky), and kill their offspring by gum secretion in oviposition scars. This plant has the potential to be used as a dead-end trap tree for ALB management. However, there is a limited understanding of the attraction ability and biochemical defense response of to ALB. In this study, we conducted host selection experiments with ALB and then performed physiological and biochemical assays on twigs from different tree species before and after ALB feeding. We analyzed the differential metabolites using the liquid chromatograph-mass spectrometer method. The results showed that ALB's feeding preference was > var. > var. . After ALB feeding, the content of soluble sugars, soluble proteins, flavonoids, and tannins decreased significantly in all species. In three comparison groups, a total of 492 differential metabolites were identified (:195, var. :255, var. :244). Differential metabolites were divided into overlapping and specific metabolites for analysis. The overlapping differential metabolites 7-isojasmonic acid, zerumbone, and salicin in the twigs of three tree species showed upregulation after ALB feeding. The specific metabolites silibinin, catechin, and geniposide, in significantly increased after being damaged. Differential metabolites enriched in KEGG pathways indicated that ALB feeding activated tyrosine metabolism and the biosynthesis of phenylpropanoids in three tree species, with a particularly high enrichment of differential metabolites in the flavonoid biosynthesis pathway in . This study provides the metabolic defense strategies of different tree species against ALB feeding and proposes candidate metabolites that can serve as metabolic biomarkers, potentially offering valuable insights into using as a control measure against ALB.
L. 能够吸引成年亚洲长角天牛（ALB），（莫氏），并通过在产卵疤痕处分泌树胶杀死其后代。这种植物有潜力被用作管理ALB的终结诱捕树。然而，对于L. 对ALB的吸引能力和生化防御反应的了解有限。在本研究中，我们对ALB进行了寄主选择实验，然后对不同树种的嫩枝在ALB取食前后进行了生理生化测定。我们使用液相色谱 - 质谱法分析了差异代谢物。结果表明，ALB的取食偏好为L. > var. > var. 。ALB取食后，所有树种中可溶性糖、可溶性蛋白、黄酮类化合物和单宁的含量均显著下降。在三个比较组中，共鉴定出492种差异代谢物（L.：195种，var. ：255种，var. ：244种）。将差异代谢物分为重叠和特异性代谢物进行分析。三种树种嫩枝中的重叠差异代谢物7 - 异茉莉酸、姜烯酮和水杨苷在ALB取食后上调。L. 中特异性代谢物水飞蓟宾、儿茶素和栀子苷在受损后显著增加。KEGG通路中富集的差异代谢物表明，ALB取食激活了三种树种中的酪氨酸代谢和苯丙烷类生物合成，L. 中黄酮类生物合成途径中的差异代谢物富集尤为明显。本研究提供了不同树种对ALB取食的代谢防御策略，并提出了可作为代谢生物标志物的候选代谢物，可能为利用L. 作为防治ALB的措施提供有价值的见解。
