School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, Guangdong, 510006, China.
Chembiochem. 2023 Jun 1;24(11):e202200767. doi: 10.1002/cbic.202200767. Epub 2023 May 8.
Enzyme fusion, the fusion of enzymes with different domains to a single protein, has been widely recognized as a promising strategy in the development of biocatalysts. Nature has evolved gene fusion to combine different catalytic enzymes to function as a fusion enzyme, and this strategy is utilized in many natural product biosynthetic pathways. Owing to rapid advances in genome sequencing and biosynthetic pathway characterization, there is increasing interest in fusion enzymes from fungal biosynthetic pathways, particularly those involved in tailoring steps. This concept aims to provide an up-to-date overview of fusion enzymes that catalyze tailoring reactions in the biosynthesis of fungal secondary metabolites. Since fungal fusion enzymes are often associated with novel metabolites, this pioneering work may stimulate the exploration of the structural diversity of fungal natural products through genome mining of the untapped biosynthetic pathways involving fusion enzymes.
酶融合，即将不同结构域的酶融合到单个蛋白质中，已被广泛认为是开发生物催化剂的一种有前途的策略。自然界已经进化出基因融合，将不同的催化酶组合在一起形成融合酶，这种策略被广泛应用于许多天然产物生物合成途径中。由于基因组测序和生物合成途径表征的快速发展，人们对真菌生物合成途径中的融合酶越来越感兴趣，特别是那些参与修饰步骤的融合酶。本综述旨在提供一个最新的概述，介绍催化真菌次生代谢产物生物合成中修饰反应的融合酶。由于真菌融合酶通常与新型代谢物相关，这项开拓性工作可能会通过对涉及融合酶的未开发生物合成途径进行基因组挖掘，来刺激对真菌天然产物结构多样性的探索。
