增加脐血和牙髓来源间充质干细胞对 HT-29 的凋亡作用。

Increasing Apoptotic Effect of Cord Blood and Wharton's Jelly-derived Mesenchymal Stem Cells on HT-29.

机构信息

Department of Medical Biology, Faculty of Medicine, Istanbul University, Istanbul, Turkey.

Institute of Graduate Studies in Health Science, Istanbul University, Istanbul, Turkey.

出版信息

Curr Stem Cell Res Ther. 2023;18(8):1133-1141. doi: 10.2174/1574888X18666230216143416.

DOI:10.2174/1574888X18666230216143416

PMID:36797607

Abstract

BACKGROUND

Colorectal cancer (CRC) is the third most common cancer worldwide. Recently, mesenchymal stem cells (MSCs) have been considered a suitable cell therapy option for cancer due to their high migration rate to the tumor site.

OBJECTIVES

The study aimed to compare the effects of human umbilical cord blood derived-MSC (UCMSC) and human Wharton's Jelly derived-MSC (WJ-MSC) on the HT-29 cell line.

METHODS

UC-MSC was obtained by Ficoll-Paque density gradient and WJ-MSC by explant method. The characterizations of MSCs and apoptosis assays were performed by flow cytometry, and caspase-3 protein levels were measured by ELISA.

RESULTS

After 72 hours of HT-29 cancer cells incubation, it was indicated that WJ-MSC was more effective at 1:5 and 1:10 ratios. Similar results were found for caspase-3 by ELISA. Moreover, WJ-MSC (1:5, < 0.006; 1:10, < 0.007) was found to be more effective at both doses compared to UC-MSC.

CONCLUSION

In this study, we used two different MSC sources at two different ratios to evaluate the apoptotic effect of MSC on HT-29 CRC cells. As a result, WJ-MSC indicated a more apoptotic effect on HT-29 cells compared to CB-MSC. We anticipated that this preliminary study would be extended in future studies. Moreover, investigating the behavior of MSC in colorectal tumor microenvironment will be beneficial for the stem cell therapy approach.

摘要

背景

结直肠癌（CRC）是全球第三大常见癌症。最近，间充质干细胞（MSCs）由于其向肿瘤部位的高迁移率，已被认为是一种合适的细胞治疗选择。

目的

本研究旨在比较人脐带来源间充质干细胞（UCMSC）和人牙髓来源间充质干细胞（WJ-MSC）对 HT-29 细胞系的影响。

方法

通过 Ficoll-Paque 密度梯度法获得 UC-MSC，通过组织块法获得 WJ-MSC。通过流式细胞术进行 MSC 特征鉴定和凋亡检测，通过 ELISA 检测 caspase-3 蛋白水平。

结果

在 HT-29 癌细胞孵育 72 小时后，发现 WJ-MSC 在 1:5 和 1:10 比例下更有效。ELISA 也得到了类似的 caspase-3 结果。此外，与 UC-MSC 相比，WJ-MSC（1:5，<0.006；1:10，<0.007）在两种剂量下均更有效。

结论

在这项研究中，我们使用两种不同的 MSC 来源和两种不同的比例来评估 MSC 对 HT-29 CRC 细胞的凋亡作用。结果表明，与 CB-MSC 相比，WJ-MSC 对 HT-29 细胞表现出更强的凋亡作用。我们预计这项初步研究将在未来的研究中得到扩展。此外，研究 MSC 在结直肠肿瘤微环境中的行为将有助于干细胞治疗方法的发展。