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肾小管细胞来源的条件培养基对大鼠糖尿病肾病损伤后微小RNA - 377、微小RNA - 29a、水通道蛋白 - 1的表达、生化及组织病理学参数的治疗作用

Therapeutic Effect of Kidney Tubular Cells-Derived Conditioned Medium on the Expression of MicroRNA-377, MicroRNA-29a, Aquapurin-1, Biochemical, and Histopathological Parameters Following Diabetic Nephropathy Injury in Rats.

作者信息

Mansouri Esrafil, Orazizadeh Mahmoud, Mard Seyyed Ali, Gorji Armita Valizadeh, Rashno Mohammad, Fakhredini Fereshtesadat

机构信息

Department of Anatomical Sciences, Faculty of Medicine, Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

Physiology Research Center, Research Institute for Infectious Diseases of Digestive System, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

出版信息

Adv Biomed Res. 2022 Dec 26;11:119. doi: 10.4103/abr.abr_375_21. eCollection 2022.

DOI:10.4103/abr.abr_375_21
PMID:36798914
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9926036/
Abstract

BACKGROUND

Diabetic nephropathy (DN) is a critical complication of diabetes mellitus. This study evaluates whether administration of conditioned medium from kidney tubular cells (KTCs-CM) has the ability to be efficacious as an alternative to cell-based therapy for DN.

MATERIALS AND METHODS

CM of rabbit kidney tubular cells (RK13; KTCs) has been collected and after centrifugation, filtered with 0.2 filters. Four groups of rats have been utilized, including control, DN, DN treated with CM, and sham group. After diabetes induction by streptozotocin (50 mg/kg body weight) in rats, 0.8 ml of the CM was injected to each rat three times per day for 3 consecutive days. Then, 24-h urine protein, blood urea nitrogen (BUN), and serum creatinine (Scr) have been measured through detection kits. The histopathological effects of CM on kidneys were evaluated by periodic acid-Schiff staining and the expression of microRNAs (miRNAs) 29a and 377 by using the real-time polymerase chain reaction. The expression of aquapurin-1 (AQP1) protein was also examined by Western blotting.

RESULTS

Intravenous injections of KTCs-CM significantly reduced the urine volume, protein 24-h, BUN, and Scr, decreased the miRNA-377, and increased miRNA-29a and AQP1 in DN treated with CM rats.

CONCLUSION

KTCs-CM may have the potential to prevent kidney injury from diabetes by regulating the microRNAs related to DN and improving the expression of AQP1.

摘要

背景

糖尿病肾病（DN）是糖尿病的一种关键并发症。本研究评估给予肾小管细胞条件培养基（KTCs-CM）作为DN细胞治疗替代方法是否有效。

材料与方法

收集兔肾小管细胞（RK13；KTCs）的条件培养基，离心后用0.2滤器过滤。使用了四组大鼠，包括对照组、DN组、用CM治疗的DN组和假手术组。大鼠经链脲佐菌素（50mg/kg体重）诱导糖尿病后，每天给每只大鼠注射0.8ml的CM，连续3天，每天3次。然后，通过检测试剂盒测量24小时尿蛋白、血尿素氮（BUN）和血清肌酐（Scr）。通过过碘酸-希夫染色评估CM对肾脏的组织病理学影响，并使用实时聚合酶链反应评估微小RNA（miRNA）29a和377的表达。还通过蛋白质印迹法检测水通道蛋白-1（AQP1）蛋白的表达。

结果

静脉注射KTCs-CM可显著降低CM治疗的DN大鼠的尿量、24小时蛋白、BUN和Scr，降低miRNA-377水平，并增加miRNA-29a和AQP1的表达。

结论

KTCs-CM可能通过调节与DN相关的微小RNA并改善AQP1的表达，具有预防糖尿病肾损伤的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e54e/9926036/6a3c907ddf14/ABR-11-119-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e54e/9926036/ada04609799b/ABR-11-119-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e54e/9926036/2972a303d902/ABR-11-119-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e54e/9926036/f1053de6c96b/ABR-11-119-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e54e/9926036/f02c61221899/ABR-11-119-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e54e/9926036/6a3c907ddf14/ABR-11-119-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e54e/9926036/ada04609799b/ABR-11-119-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e54e/9926036/2972a303d902/ABR-11-119-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e54e/9926036/f1053de6c96b/ABR-11-119-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e54e/9926036/f02c61221899/ABR-11-119-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e54e/9926036/6a3c907ddf14/ABR-11-119-g005.jpg

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