College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, China.
Department of Otorhinolaryngology, Head & Neck Surgery, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, China.
J Mater Chem B. 2023 Mar 8;11(10):2266-2275. doi: 10.1039/d2tb02608b.
The applications of fluorescence imaging in tumor detection and assistance in tumor resection have become progressively more widespread. Biocompatible fluorescent nanoparticles with high sensitivity and selectivity are a challenge for biological fluorescence imaging. Ligand-mediated targeting of nanoparticles to tumors is an appealing tactic for improving imaging efficiency. Herein, tetraphenyl ethylene (TPE) and phenylboronic acid (PBA) were introduced into polyurethane to synthesize a PU-TPE-PBA (PTP) fluorescent emulsion with aggregation-induced emission (AIE) for targeted tumor imaging. The PTP emulsion with a size of less than 50 nm shows excellent stability and high fluorescence sensitivity (extremely low TPE concentrations of 0.31 μg mL). Since PBA can selectively recognize and bind to sialic acid (SA) which is widely overexpressed in tumor cells, such PTP nanoparticles can be enriched in tumors and retained for longer periods due to enhanced permeability and retention (EPR) as well as active targeting effects. In addition, the PTP emulsion exhibits good biocompatibility and biosafety. Therefore, the novel PTP emulsion is promising for tumor cell imaging.
荧光成像在肿瘤检测和辅助肿瘤切除中的应用已经越来越广泛。具有高灵敏度和选择性的生物相容性荧光纳米粒子是生物荧光成像的一个挑战。配体介导的纳米粒子靶向肿瘤是提高成像效率的一种有吸引力的策略。在此，将四苯基乙烯（TPE）和苯硼酸（PBA）引入到聚氨酯中，合成了具有聚集诱导发射（AIE）的用于靶向肿瘤成像的荧光乳液 PU-TPE-PBA（PTP）。粒径小于 50nm 的 PTP 乳液具有极好的稳定性和高荧光灵敏度（TPE 的浓度极低，为 0.31μg/mL）。由于 PBA 可以选择性地识别和结合在肿瘤细胞中广泛过表达的唾液酸（SA），因此由于增强的通透性和保留（EPR）以及主动靶向作用，这种 PTP 纳米粒子可以在肿瘤中富集并保持更长时间。此外，PTP 乳液具有良好的生物相容性和生物安全性。因此，新型 PTP 乳液有望用于肿瘤细胞成像。
