School of Geographical Science, Fujian Normal University, Fuzhou 350007, China.
Cultivation Base of State Key Laboratory of Humid Subtropical Mountain Ecology, Fuzhou 350007, China.
Ying Yong Sheng Tai Xue Bao. 2023 Jan;34(1):235-241. doi: 10.13287/j.1001-9332.202212.034.
To understand the effects of common afforestation tree species on soil microbial community in subtropical forests, seven different tree species were selected as the research object, including , , , , , and . Based on 16S rRNA high-throughput sequencing and real-time quantitative PCR techniques, we explored the effects of different tree species on soil bacterial community composition, diversity and microbial functional guilds. The results showed that Acidobacteria, Proteobacteria, and Actinobacteria were the dominant bacterial phyla, and that there was no significant difference in bacterial diversity or richness index among different tree species. Results of redundancy analysis suggested that soil bulk density, soil C/N, litter nitrogen content, and litter C/N were the predominant factors determining soil bacterial community composition. The afforestation tree species had significant effects on functional gene abundances of ammonia oxidizing archaea, ammonia oxidizing bacteria and complete ammonia oxidation. Comammox were dominant in abundance. Ammonia oxidizing archaea gene was the only type whose abundance showed significant correlation with soil nitrate content, suggesting that ammonia oxidizing archaea could play a dominant role in the autotrophic nitrification in the acidic subtropical forest soils. The afforestation tree species had significant effects on functional gene abundances of ammonia oxidizing microorganisms. Results of correlation analysis showed that litter nitrogen content was the driving factor for the abundance of ammonia oxidizing microorganisms. Our study provided strong evidence that the responses of soil microbial functional guilds to tree species were more sensitive than bacterial community composition. Future studies should explore the mechanisms of tree plantations on forest ecosystem functioning from the perspective of microbial functional guilds.
为了了解常见造林树种对亚热带森林土壤微生物群落的影响，选择了 7 种不同的树种作为研究对象，包括 、 、 、 、 、 。基于 16S rRNA 高通量测序和实时定量 PCR 技术，我们探讨了不同树种对土壤细菌群落组成、多样性和微生物功能类群的影响。结果表明，酸杆菌门、变形菌门和放线菌门是主要的细菌门，不同树种之间的细菌多样性或丰富度指数没有显著差异。冗余分析的结果表明，土壤容重、土壤 C/N、凋落物氮含量和凋落物 C/N 是决定土壤细菌群落组成的主要因素。造林树种对氨氧化古菌、氨氧化细菌和全程氨氧化功能基因丰度有显著影响。其中，共氨氧化菌占主导地位。氨氧化古菌基因是唯一与土壤硝酸盐含量呈显著相关的基因，表明氨氧化古菌可能在酸性亚热带森林土壤的自养硝化过程中发挥主导作用。造林树种对氨氧化微生物的功能基因丰度有显著影响。相关分析的结果表明，凋落物氮含量是氨氧化微生物丰度的驱动因素。本研究为土壤微生物功能类群对树种的响应比细菌群落组成更为敏感提供了有力证据。未来的研究应从微生物功能类群的角度探讨人工林对森林生态系统功能的影响机制。
