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红外辐射温度在慢性心肌缺血损伤大鼠穴位体表的特异性表达研究。

Study on the Specific Expression of Infrared Radiation Temperature on the Body Surface of Acupoint in Rats with Chronic Myocardial Ischemic Injury.

机构信息

Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, 610075, P.R. China.

Shanxi University of Traditional Chinese Medicine, Shanxi, Taiyuan, 030002, P.R. China.

出版信息

Curr Med Imaging. 2023;19(13):1580-1590. doi: 10.2174/1573405619666230217120343.

Abstract

BACKGROUND

Infrared thermal imaging technology was used to observe the changes in infrared radiation temperature at acupoints in rats caused by chronic myocardial ischemia injury.

OBJECTIVE

This study aims to compare the difference of body surface infrared radiation temperature information of three groups of acupoints: bilateral Neiguan (PC6), bilateral Yanglingquan (GB33), and bilateral Sham Acupoints (SA) in the pathological state of myocardial ischemia injury, and to explore the relationship between acupoints and viscera state.

METHODS

SPF adult Wistar male rats (n = 20) were randomly divided into a control (CTL; n = 10) and an isoproterenol group (ISO; n = 10). Chronic myocardial injury was induced in rats by subcutaneous injection of isoproterenol hydrochloride for 14 d. On the second day after the establishment of the model, the serum levels of cardiac troponin (cTnI) and creatine kinase isoenzyme (CK-MB) were measured by enzyme-linked immunosorbent assay (ELISA). The morphological changes of the myocardial tissue in the two groups were observed by hematoxylin-eosin (HE) staining and their pathological scores were evaluated, which was then used to determine the myocardial ischemic injury. Two days before and after the establishment of the model, the electrocardiograms (ECG) of the two groups of rats were recorded by the (ECG) data acquisition system, and the infrared thermal imaging platform was used to detect the temperature of the six acupoints.

RESULTS

  1. After subcutaneous injection of isoproterenol hydrochloride for 14 days, the ST segment of the ECG decreased in the ISO group compared with that of the CTL group; 2. Myocardial tissue injury was serious in the ISO group compared to the CTL group; 3. Serum cTn-I and CK-MB were significantly increased (P <0 01) in the ISO group, compared to that in the CTL group; 4. The infrared radiation temperature on the body surface of bilateral Neiguan (PC6) acupoints decreased significantly in the ISO group, compared to that of the CTL group.

CONCLUSION

Infrared thermal imaging technology can be used to detect the changes in the energy state of acupoints. Chronic myocardial ischemic injury can cause a decrease in IR temperature on the body surface of bilateral Neiguan (PC6) acupoints, suggesting that visceral diseases can lead to changes in the energy metabolism of acupoints.

摘要

背景

红外热成像技术用于观察慢性心肌缺血损伤大鼠穴位红外辐射温度的变化。

目的

本研究旨在比较三组穴位(双侧内关 PC6、双侧阳陵泉 GB33 和双侧假穴 SA)在心肌缺血损伤病理状态下体表红外辐射温度信息的差异,探讨穴位与脏腑状态的关系。

方法

SPF 成年雄性 Wistar 大鼠(n=20)随机分为对照组(CTL;n=10)和异丙肾上腺素组(ISO;n=10)。通过皮下注射盐酸异丙肾上腺素连续 14d 诱导大鼠慢性心肌损伤。模型建立后第 2 天,采用酶联免疫吸附法(ELISA)测定血清心肌肌钙蛋白(cTnI)和肌酸激酶同工酶(CK-MB)水平。采用苏木精-伊红(HE)染色观察两组心肌组织形态变化,评价其病理评分,从而确定心肌缺血损伤。模型建立前后两天,采用心电图(ECG)数据采集系统记录两组大鼠 ECG,采用红外热成像平台检测六穴温度。

结果

  1. 皮下注射盐酸异丙肾上腺素 14d 后,ISO 组大鼠 ECG 的 ST 段较 CTL 组降低;2. ISO 组大鼠心肌组织损伤较 CTL 组严重;3. ISO 组大鼠血清 cTn-I 和 CK-MB 明显升高(P<0.01),较 CTL 组;4. ISO 组双侧内关(PC6)穴位体表红外辐射温度明显降低。

结论

红外热成像技术可用于检测穴位能量状态的变化。慢性心肌缺血损伤可导致双侧内关(PC6)穴位体表 IR 温度降低,提示内脏疾病可导致穴位能量代谢变化。

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