Hebei Province Key Laboratory of Study and Exploitation of Chinese Medicine, Chengde Medical University, Chengde, Heibei 067000, China.
Hebei Key Laboratory of Nerve Injury and Repair, Chengde Medical University, Chengde, Heibei 067000, China.
J AOAC Int. 2023 Jul 17;106(4):1017-1036. doi: 10.1093/jaoacint/qsad024.
Xiangsha Pingwei Pills (XPP) is a traditional Chinese medicine (TCM) prescription, which is widely used to treat epigastric pain in China. Its systematic chemical characteristics have rarely been reported, which hinders the interpretation of the material basis of its prescription.
To establish a rapid and effective component characterization method for XPP using ultra-HPLC-quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS) and the data post-processing program, Peakview 1.2 software.
A UPLC-Q-TOF-MS method coupled with Peakview 1.2 software was successfully established for the first time to investigate the complex constituents of XPP. Accurate MS and MS/MS data were detected in positive and negative ion mode. The compounds were tentatively identified based on their retention times, MS, and MS/MS data, as well as reference standards and from the literature.
The chemical profile of XPP was acquired, and a total of 130 compounds in XPP were preliminarily identified for the first time, including 6 organic acids, 59 flavonoids, 13 lignans, 20 terpenoids, 9 phenylpropanoids, 6 alkaloids, 5 amino acids, and 12 other compounds.
A rapid and effective UPLC-Q-TOF-MS method for the main chemical components of XPP has been established for further characterizing constituents and the overall quality control of XPP.
This is the first report of a comprehensive analysis method for the main chemical components of XPP, which aims to lay a solid foundation for the chemical basis and overall quality control of XPP.
香砂平胃丸（XPP）是一种中药方剂，在中国被广泛用于治疗胃脘痛。但其系统的化学特征鲜有报道，这阻碍了对其方剂物质基础的阐释。
首次采用超高效液相色谱-四极杆飞行时间串联质谱法（UPLC-Q-TOF-MS）联合 Peakview 1.2 软件建立一种快速、有效的 XPP 成分特征分析方法。
首次建立了 UPLC-Q-TOF-MS 方法结合 Peakview 1.2 软件，用于研究 XPP 的复杂成分。采用正、负离子模式检测精确的 MS 和 MS/MS 数据。基于保留时间、MS 和 MS/MS 数据以及对照品和文献，对化合物进行了初步鉴定。
首次获得了 XPP 的化学成分图谱，共初步鉴定出 XPP 中的 130 种化合物，包括 6 种有机酸、59 种黄酮类化合物、13 种木脂素类、20 种萜类化合物、9 种苯丙素类、6 种生物碱、5 种氨基酸和 12 种其他化合物。
建立了一种快速、有效的 XPP 主要化学成分的 UPLC-Q-TOF-MS 分析方法，旨在为 XPP 的化学成分基础和整体质量控制奠定基础。
这是首次对 XPP 主要化学成分进行全面分析方法的报道，旨在为 XPP 的化学基础和整体质量控制奠定基础。
