Kurobane I, Dale P L, Vining L C
Department of Biology, Dalhousie University, Halifax, N.S., Canada.
J Antibiot (Tokyo). 1987 Aug;40(8):1131-9. doi: 10.7164/antibiotics.40.1131.
Cultures of Streptomyces griseus grown under phosphate-limiting conditions produced a complex of green products. Three of these were separated from the mixture and characterized. One was identified as viridomycin A, the ferrous chelate of 4-hydroxy-3-nitrosobenzaldehyde; the second (actinoviridin A) was the corresponding carboxylic acid chelate and the third (viridomycin E) was a hybrid chelate containing both the aldehyde and acid ligands. Only two out of nine strains of S. griseus examined produced viridomycins and the ligands were biosynthesized only in media from which phosphate had been exhausted. Optimization of the production medium showed that fructose and alanine were the most favorable carbon and nitrogen sources and that relatively high concentrations of ferrous ions were necessary. The results suggest that viridomycins are not produced by S. griseus as iron scavengers in response to iron deficiency but as secondary metabolites that are stabilized adventitiously in the broth by metal ion chelation.
在磷酸盐限制条件下培养的灰色链霉菌产生了一种绿色产物复合物。其中三种从混合物中分离出来并进行了表征。一种被鉴定为绿霉素A，即4-羟基-3-亚硝基苯甲醛的亚铁螯合物；第二种（放线绿菌素A）是相应的羧酸螯合物，第三种（绿霉素E）是一种同时含有醛和酸配体的混合螯合物。在所检测的九株灰色链霉菌中，只有两株产生绿霉素，且配体仅在磷酸盐已耗尽的培养基中生物合成。生产培养基的优化表明，果糖和丙氨酸是最有利的碳源和氮源，并且需要相对高浓度的亚铁离子。结果表明，灰色链霉菌产生绿霉素并非作为应对缺铁的铁清除剂，而是作为次生代谢产物，通过金属离子螯合偶然在肉汤中稳定下来。
