Department of Zoology, Microbial Biotechnology and Vermi-Technology Laboratory, Vermi-tech Unit, University of Azad Jammu and Kashmir, Muzaffarabad, Pakistan.
First Biotech LLC, Lahore, Pakistan.
PLoS One. 2023 Feb 17;18(2):e0279828. doi: 10.1371/journal.pone.0279828. eCollection 2023.
The current research was conducted at Vermi-tech Unit, Muzaffarabad in 2018 to evaluate the efficacy of cow dung and vermicompost on seed sprouting, seedlings, and vegetative developmental parameters of Viola x wittrokiana (pansy). In the current study, vermicompost was produced using Eisenia fetida. Physicochemical parameters of vermicompost and organic manure were recorded before each experimentation. The potting experiment was designed and comprised of eight germination mediums containing different combinations of soil, sand, cow dung, and various concentrations of vermicompost such as 10% VC, 15% VC, 20% VC, 25% VC, 30% VC, and 35% VC. Seed sprouting and seedling developmental parameters were observed for 28 days while vegetative plant growth parameters were recorded after 10 weeks of transplantation in various vermicompost amended germination media. Pre and post-physicochemical analysis of germination media were also recorded to check their quality and permanency. The current findings showed that 30% VC germination media was an effective dose for early seed germination initiation and all seed germination parameters. However, the significant vegetative plant growth and flowering parameters of pansy occurred at 35% VC. Findings revealed that vermicompost not only enhanced the seed germination and growth of pansy but also improved soil health. These results indicate that vermicompost can be exploited as a potent bio-fertilizer for ornamental plant production.
本研究于 2018 年在 Muzaffarabad 的 Vermi-tech 单位进行,旨在评估牛粪和蚯蚓粪对 Viola x wittrokiana(三色堇)种子萌发、幼苗和营养生长发育参数的功效。在本研究中,使用赤子爱胜蚓生产了蚯蚓粪。在每次实验前,记录了蚯蚓粪和有机肥的理化参数。设计了盆栽实验,包含 8 种发芽培养基,其中含有不同组合的土壤、沙子、牛粪和不同浓度的蚯蚓粪,如 10% VC、15% VC、20% VC、25% VC、30% VC 和 35% VC。观察了 28 天的种子萌发和幼苗发育参数,在不同浓度的蚯蚓粪改良发芽培养基中移栽 10 周后记录了营养生长植物生长参数。还记录了发芽培养基的理化分析前后数据,以检查其质量和稳定性。目前的研究结果表明,30% VC 的发芽培养基是促进种子早期萌发和所有种子萌发参数的有效剂量。然而,三色堇的显著营养生长和开花参数出现在 35% VC 中。研究结果表明,蚯蚓粪不仅能促进三色堇种子的萌发和生长,还能改善土壤健康。这些结果表明,蚯蚓粪可用作观赏植物生产的有效生物肥料。