Chaieb Kamel, Kouidhi Bochra, Ayed Lamia, Bakr Hosawi Salman, Abdulbaqi Abdulhakim Jawaher, Hajri Azhar, Altayb Hisham N
Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
Laboratory of Analysis, Treatment and Valorization of Pollutants of the Environmental and Products, Faculty of Pharmacy, University of Monastir, Tunisia.
Bioresour Technol. 2023 Apr;374:128755. doi: 10.1016/j.biortech.2023.128755. Epub 2023 Feb 17.
In this study, Box-Behnken design combining seven factors at three levels were used to optimize the elimination of CI Reactive Red 66 in artificial seawater, by the combination of eco-friendly bio-sorbents and acclimated halotolerant microbial strain. Results showed that macro-algae and cuttlebone (2 %) were the best natural bio-sorbent. Additionally, the selected halotolerant strain able to rapidly remove dye was identified as Shewanella algae B29. The optimization process revealed that decolourization of CI Reactive Red 66 yields reached 91.04 % under the following variable values: dyes concentration (100 mg/l), salinity (30 g/l), peptone (2 %), pH (5), algae C (3 %), cuttlebone (1.5 %) and agitation (150 rpm). The whole genome analysis of S. algae B29 demonstrated the presence of several genes coding for valuable enzymes involved in textile dyes biotransformation, adaptation to stress as well as biofilm formation implying its potential use in biological textile wastewater treatment.
在本研究中，采用三水平七因素的Box-Behnken设计，通过结合环保型生物吸附剂和驯化的耐盐微生物菌株，优化人工海水中活性艳红66的去除效果。结果表明，大型海藻和乌贼骨（2%）是最佳的天然生物吸附剂。此外，筛选出的能够快速去除染料的耐盐菌株被鉴定为嗜藻希瓦氏菌B29。优化过程表明，在以下变量值下，活性艳红66的脱色率达到91.04%：染料浓度（100mg/l）、盐度（30g/l）、蛋白胨（2%）、pH值（5）、海藻C（3%）、乌贼骨（1.5%）和搅拌速度（150rpm）。嗜藻希瓦氏菌B29的全基因组分析表明，存在几个编码参与纺织染料生物转化、应激适应以及生物膜形成的有价值酶的基因，这意味着其在生物纺织废水处理中的潜在应用价值。
