Federal University of São João del-Rei, Natural Science Department, CEP 36301160, São João del-Rei, MG, Brazil.
Federal University of Juiz de Fora, Chemistry Department, Institute of Exact Sciences, CEP 36036-900, Juiz de Fora, MG, Brazil.
Carbohydr Res. 2023 Mar;525:108762. doi: 10.1016/j.carres.2023.108762. Epub 2023 Feb 10.
In order to enable the applicability of chitosan as an antifungal, soil fungi were isolated and identified, then used in its production. Fungal chitosan has several advantages, including lower toxicity, low cost, and high degree of deacetylation. These characteristics are essential for therapeutic applications. The results indicate high viability of the isolated strains to produce chitosan, obtaining a maximum yield of 40.59 mg chitosan/g of dry biomass. M. pseudolusitanicus L. was reported for the first time for production by chitosan. The chitosan signals were observed by ATR-FTIR and C SSNMR. Chitosans showed high degrees of deacetylation (DD), ranging from 68.8% to 88.5%. In comparison with the crustacean chitosan, Rhizopus stolonifer and Cunninghamella elegans presented lower viscometric molar masses (26.23 and 22.18 kDa). At the same time, the molar mass of chitosan Mucor pseudolusitanicus L. showed a value coincident with that assumed as low molar mass (50,000-150,000 g mol). Concerning the in vitro antifungal potential against the dermatophyte fungus Microsporum canis (CFP 00098), the fungal chitosans showed satisfactory antifungal activities, inhibiting mycelial growth by up to 62.81%. This study points to the potential of chitosans extracted from fungal cell walls for applications in the inhibition of the growth of (Microsporum canis) human pathogenic dermatophyte.
为了使壳聚糖能够作为一种抗真菌剂得到应用，我们对土壤真菌进行了分离和鉴定，并将其用于壳聚糖的生产。真菌壳聚糖具有一些优点，包括毒性较低、成本较低和脱乙酰度较高。这些特性对于治疗应用至关重要。结果表明，分离株具有较高的壳聚糖生产活力，可获得最高 40.59 mg 壳聚糖/g 干生物质的产率。首次报道了 M. pseudolusitanicus L. 可用于生产壳聚糖。壳聚糖信号通过 ATR-FTIR 和 C SSNMR 进行观察。壳聚糖的脱乙酰度（DD）很高，范围为 68.8%至 88.5%。与甲壳质壳聚糖相比，Rhizopus stolonifer 和 Cunninghamella elegans 的特性粘度摩尔质量较低（26.23 和 22.18 kDa）。同时，Mucor pseudolusitanicus L. 壳聚糖的摩尔质量显示出与低摩尔质量（50,000-150,000 g mol）一致的值。关于对亲动物性真菌 Microsporum canis（CFP 00098）的体外抗真菌潜力，真菌壳聚糖表现出令人满意的抗真菌活性，可抑制菌丝生长高达 62.81%。本研究表明，从真菌细胞壁中提取的壳聚糖在抑制（Microsporum canis）人致病性亲动物性真菌的生长方面具有潜力。
