Zou Wenxue, Huang Rui, Li Peihang, Liu Xiang, Huang Qingyu, Yue Jinbo, Liu Chao
Department of Radiation Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, Shandong, China.
Department of Radiation Oncology, Tianjin Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, National Clinical Research Center for Cancer, Tianjin Medical University Cancer Institute and Hospital, Tianjin, China.
J Infect Public Health. 2023 Apr;16(4):542-550. doi: 10.1016/j.jiph.2023.01.019. Epub 2023 Jan 31.
To investigate T cell immunoreceptor with Ig and ITIM domain (TIGIT), V-domain Ig suppressor of T cell activation (VISTA), and lymphocyte-activation gene-3 (LAG-3) expression in pathological tissue of human papillomavirus (HPV)-infected cervical cancer (CC) patients and their relationship with patient prognosis.
Clinical data of 175 patients with HPV-infected CC were collected retrospectively. Tumor tissue sections were stained immunohistochemically for TIGIT, VISTA, and LAG-3. The Kaplan-Meier method calculated patient survival. Univariate and multivariate Cox proportional hazards models analyzed all potential risk factors for survival.
When combined positive score (CPS)= 1 was used as the cut-off value, the Kaplan-Meier survival curve showed that the progression-free survival (PFS) and overall survival (OS) of patients with positive expression of TIGIT and VISTA are shorter (both p < 0.05). Univariate COX regression analysis showed that the positive expression of TIGIT and VISTA are related to patient PFS and OS (both HR>1.0 and p < 0.05). Multivariate COX regression analysis showed that TIGIT-positive patients had shorter OS and VISTA-positive patients had shorter PFS (both HR>1.0 and p < 0.05). There is no significant correlation between LAG-3 expression and PFS or OS. When CPS= 10 was used as the cut-off value, Kaplan-Meier survival curve showed that TIGIT-positive patients had shorter OS (p = 0.019). Univariate COX regression analysis showed that TIGIT-positive expression was associated with the OS of patients (HR=2.209, CI: 1.118-4.365, p = 0.023). However, multivariate COX regression analysis showed that TIGIT expression was not associated significantly with OS. There was no significant correlation between VISTA and LAG-3 expression and PFS or OS.
TIGIT and VISTA are associated closely with HPV-infected CC prognosis and are effective biomarkers.
探讨人乳头瘤病毒（HPV）感染的宫颈癌（CC）患者病理组织中T细胞免疫受体Ig和免疫受体酪氨酸抑制基序结构域（TIGIT）、T细胞活化V结构域Ig抑制分子（VISTA）和淋巴细胞活化基因3（LAG-3）的表达及其与患者预后的关系。
回顾性收集175例HPV感染的CC患者的临床资料。肿瘤组织切片进行TIGIT、VISTA和LAG-3免疫组织化学染色。采用Kaplan-Meier法计算患者生存率。单因素和多因素Cox比例风险模型分析所有生存的潜在危险因素。
以联合阳性评分（CPS）=1为临界值时，Kaplan-Meier生存曲线显示，TIGIT和VISTA阳性表达患者的无进展生存期（PFS）和总生存期（OS）较短（均p<0.05）。单因素COX回归分析显示，TIGIT和VISTA的阳性表达与患者的PFS和OS相关（均HR>1.0且p<0.05）。多因素COX回归分析显示，TIGIT阳性患者的OS较短，VISTA阳性患者的PFS较短（均HR>1.0且p<0.05）。LAG-3表达与PFS或OS之间无显著相关性。以CPS=10为临界值时，Kaplan-Meier生存曲线显示，TIGIT阳性患者的OS较短（p=0.019）。单因素COX回归分析显示，TIGIT阳性表达与患者的OS相关（HR=2.209，CI：1.118-4.365，p=0.023）。然而，多因素COX回归分析显示，TIGIT表达与OS无显著相关性。VISTA和LAG-3表达与PFS或OS之间无显著相关性。
TIGIT和VISTA与HPV感染的CC预后密切相关，是有效的生物标志物。
