College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 May 15;293:122504. doi: 10.1016/j.saa.2023.122504. Epub 2023 Feb 15.
Fluorescent bovine serum albumin-protected gold nanoclusters (BSA@Au NCs) can catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) to produce blue oxTMB for its peroxidase-like activity. The two absorption peaks of oxTMB overlapped with the excitation and emission peaks of BSA@Au NCs, respectively, causing efficient quenching on the fluorescence of BSA@Au NCs. The quenching mechanism can be attributed to the dual inner filter effect (IFE). Based on the dual IFE, BSA@Au NCs were utilized as both peroxidase mimics and fluorescent reporters for HO detection and further for uric acid detection with uricase. Under optimal detection conditions, the method can be used to detect HO ranging 0.50-50 μM with a detection limit of 0.44 μM and UA ranging 0.50-50 μM with a detection limit of 0.39 μM. The established method had been successfully utilized for the determination of UA in human urine, with massive potential in biomedical applications.
荧光牛血清白蛋白保护的金纳米簇（BSA@Au NCs）可以催化 3,3',5,5'-四甲基联苯胺（TMB）的氧化，产生蓝色的 oxTMB，因其具有过氧化物酶样活性。oxTMB 的两个吸收峰分别与 BSA@Au NCs 的激发和发射峰重叠，导致 BSA@Au NCs 的荧光被有效猝灭。猝灭机制可以归因于双重内滤效应（IFE）。基于双重 IFE，BSA@Au NCs 被用作 HO 检测的过氧化物酶模拟物和荧光报告物，并进一步与尿酸酶一起用于尿酸（UA）检测。在最佳检测条件下，该方法可用于检测 0.50-50 μM 的 HO，检测限为 0.44 μM，以及 0.50-50 μM 的 UA，检测限为 0.39 μM。该方法已成功用于人尿中 UA 的测定，在生物医学应用中具有巨大的潜力。
