Key Laboratory of Colloid and Interface Chemistry, Shandong University, Ministry of Education, Jinan, 250100, China.
Qilu University of Technology (Shandong Academy of Sciences), Shandong Analysis and Test Center, 19 Keyuan Street, Jinan, 250014, China.
Talanta. 2023 May 15;257:124344. doi: 10.1016/j.talanta.2023.124344. Epub 2023 Feb 15.
Due to the serious risks of lead pollution to human health, it plays a great role in constructing a simple, inexpensive, portable, and user-friendly strategy for Pb detection in environmental samples. Herein, a paper-based distance sensor is developed to detect Pb assisted with the target-responsive DNA hydrogel. Pb can activate DNAzyme to cleave its substrate strand, which results in the hydrolysis of the DNA hydrogel. The released water molecules trapped in the hydrogel can flow along the patterned pH paper due to the capillary force. The water flow distance (WFD) is significantly influenced by the amount of water released from the collapsed DNA hydrogel triggered by the addition of various Pb concentrations. In this way, Pb can be quantitatively detected without using specialized instruments and labeled molecules, and the limit of detection (LOD) of Pb is 3.0 nM. Additionally, the Pb sensor works well in lake water and tap water. Overall, this simple, inexpensive, portable, and user-friendly method is very promising for quantitative and in-field detection of Pb with excellent sensitivity and selectivity.
由于铅污染对人类健康的严重风险，因此构建一种简单、经济、便携且易于使用的环境样品中 Pb 检测策略具有重要意义。在此，开发了一种基于纸张的距离传感器，该传感器通过目标响应性 DNA 水凝胶来检测 Pb。Pb 可以激活 DNA 酶来切割其底物链，从而导致 DNA 水凝胶的水解。由于毛细作用力，被困在水凝胶中的释放水分子可以沿着图案化的 pH 纸流动。水流动距离 (WFD) 受到由各种 Pb 浓度引发的从坍塌的 DNA 水凝胶中释放的水量的显著影响。通过这种方式，可以在不使用专用仪器和标记分子的情况下对 Pb 进行定量检测，Pb 的检测限 (LOD) 为 3.0 nM。此外，Pb 传感器在湖水和自来水中都能很好地工作。总的来说，这种简单、经济、便携且易于使用的方法具有出色的灵敏度和选择性，非常有希望用于 Pb 的定量和现场检测。
