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LK93,一种严重导致小麦根腐病的病原体,其高质量核基因组和线粒体基因组。

High-Quality Nuclear Genome and Mitogenome of LK93, a Devastating Pathogen Causing Wheat Root Rot.

机构信息

Department of Plant Pathology, College of Plant Protection, Henan Agricultural University/Collaborative Innovation Center of Henan Grain Crops/National Key Laboratory of Wheat and Maize Crop Science, Zhengzhou 450002, China.

State Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, the Provincial Key Laboratory of Plant Pathology of Hubei Province, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan 430070, China.

出版信息

Mol Plant Microbe Interact. 2023 Jul;36(7):452-456. doi: 10.1094/MPMI-09-22-0196-A. Epub 2023 Aug 14.

Abstract

, one of the most devastating hemibiotrophic fungal pathogens, causes root rot, crown rot, leaf blotching, and black embryos of gramineous crops worldwide, posing a serious threat to global food security. However, the host-pathogen interaction mechanism between and wheat remains poorly understood. To facilitate related studies, we sequenced and assembled the genome of LK93. Nanopore long reads and next generation sequencing short reads were applied in the genome assembly, and the final 36.4-Mb genome assembly contains 16 contigs with the contig N of 2.3 Mb. Subsequently, we annotated 11,811 protein-coding genes. Of these, 10,620 were functional genes, 258 of which were identified as secretory proteins, including 211 predicted effectors. Additionally, the 111,581-bp mitogenome of LK93 was assembled and annotated. The LK93 genomes presented in this study will facilitate research in the -wheat pathosystem for better control of crop diseases. [Formula: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.

摘要

小麦茎基腐病菌是一种极具破坏性的兼性半活体真菌病原体,可导致禾谷类作物的根腐、冠腐、叶片斑点和黑胚,对全球粮食安全构成严重威胁。然而,小麦茎基腐病菌与小麦之间的互作机制仍不清楚。为了促进相关研究,我们对 LK93 进行了测序和组装。我们在基因组组装中应用了纳米孔长读长和下一代测序短读长,最终得到的 36.4Mb 基因组组装包含 16 个 contig,contig N 为 2.3Mb。随后,我们注释了 11811 个蛋白编码基因。其中 10620 个是功能基因,258 个被鉴定为分泌蛋白,包括 211 个预测的效应子。此外,还组装和注释了 LK93 的 111581bp 线粒体基因组。本研究中提供的 LK93 基因组将有助于小麦茎基腐病系统的研究,以更好地控制作物病害。[公式:见正文] 版权所有 © 2023 作者。这是一篇在 CC BY-NC-ND 4.0 国际许可下发布的开放获取文章。

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