Department of Plant Science, Macdonald Campus of McGill University, 21,111 Lakeshore Rd., Ste-Anne de Bellevue, H9X 3V9 Quebec, Canada.
Genomics. 2023 Mar;115(2):110583. doi: 10.1016/j.ygeno.2023.110583. Epub 2023 Feb 15.
Plant growth promoting rhizobacteria (PGPR) communicate with plants through roots. The molecular mechanism by which plants and PGPR respond to each other is not very well known. In the current study, we did RNA sequence analysis of Brachypodium distachyon Bd21-3 roots inoculated with PGPR, Bacillus velezensis strain B26. From our list of differentially expressed genes, we concentrated on transcripts that have a high possibility of participating in plant-PGPR interaction. Transcripts associated to the hormone signalling pathway were differentially expressed. We identified the upregulation of various transcripts linked to ion transporters. Reduction in expression of defense signalling genes indicated that B26 suppresses the plant defense mechanisms to begin successful interaction with roots. Transcripts associated with lignin branch of the phenylpropanoid pathway were upregulated as well, leading to more accumulation of lignin in the cell wall which enhances mechanical strength of plants. Overall, this study is an excellent resource for investigating associations between plant-PGPR interactions.
植物促生根际细菌（PGPR）通过根部与植物进行交流。植物和 PGPR 相互响应的分子机制还不是很清楚。在本研究中，我们对接种 PGPR、解淀粉芽孢杆菌 B26 的拟南芥 Bd21-3 根进行了 RNA 序列分析。从我们差异表达基因列表中，我们集中研究了那些极有可能参与植物- PGPR 相互作用的转录本。与激素信号通路相关的转录本表达存在差异。我们鉴定出了与离子转运体相关的各种转录本的上调。防御信号基因表达的减少表明 B26 抑制了植物防御机制，以开始与根部的成功相互作用。与苯丙烷途径的木质素分支相关的转录本也上调了，导致细胞壁中木质素的积累增加，从而增强了植物的机械强度。总的来说，这项研究是研究植物-PGPR 相互作用之间关联的极好资源。
