Henzel W J, Rodriguez H, Watanabe C
Department of Developmental Biology, Genentech, Inc., South San Francisco, CA 94080.
J Chromatogr. 1987 Aug 28;404(1):41-52. doi: 10.1016/s0021-9673(01)86835-7.
Computer programs are described that allow facile analysis of data from a protein sequencer and amino acid analyzer. The sequencer program provides automated sequence interpretation while requiring minimal user interaction. The program serves as a powerful aid in deciphering mixture sequences and allows routine monitoring of sequencer performance. The computer program for amino acid analysis data provides the following calculations: mole percent, protein concentration and residues per mole with comparison between theoretical and calculated values. A plot of molecular weight versus deviation from integer values is calculated providing a measure of peptide or protein purity.
本文描述了一些计算机程序，这些程序可方便地分析来自蛋白质测序仪和氨基酸分析仪的数据。测序仪程序可实现自动化序列解读，同时只需用户进行极少的交互操作。该程序在解读混合序列方面是一个强大的辅助工具，还能对测序仪的性能进行常规监测。用于氨基酸分析数据的计算机程序可进行以下计算：摩尔百分比、蛋白质浓度以及每摩尔的残基数，并能对理论值和计算值进行比较。通过计算分子量与偏离整数值的偏差绘制的图表，可衡量肽或蛋白质的纯度。
